Tailor every stain

  • Fast – In solution, label biotinylated antibodies with fluorochromes in less than 5 min. No washes.
  • Flexible – Tailor each stain. Efficiently label as little as 0,1 μg biotinylated antibody with no antibody loss.
  • Multiplexing – Immunostain with multiple biotinylated antibodies of any animal species. No cross reactions.

 

* * *

“The big advantage of Flexistain is the flexibility! You can easily change which fluorochrome to attach
to the biotinylated antibody and you can use several biotinylated antibodies together. Furthermore,
in the lab we are using some antibodies that are not commercially available with suitable fluorochromes.
Without FlexiStain we could not easily use these antibodies for multi-immunostaining.
Finally, immunostaining with FlexiStain is time-saving since you skip the secondary step.”

– Anneli Strömberg, researcher at University of Gothenburg

Multiplex with biotinylated antibodies              FlexiStain is a unique immunolabeling tool that enables multiplexing with several biotinylated antibodies. There is no spillover of FlexiStain between antibodies and antibodies of the same animal host species can be used.

Just click on your fluorochrome                            FlexiStain attaches fluorochromes via monovalent streptavidin to biotinylated antibodies using the streptaclick technology. The click reaction is performed in solution in less than 5 minutes with no purification step and no loss of antibodies. The reaction can be run in microliter volumes, which makes it practical to label antibodies even for single experiments with any of the available fluorochromes (AF488, Atto542, AF594, AF647). FlexStained antibodies are as bright as directly conjugated antibodies and can be used straight away or stored at +4C for use later. This makes FlexiStain a powerful tool for quick and easy multi-immunostaining.

Application:  IHC and flow cytometry

Illustrated user protocol
Step 1

Conjugation

<p><strong><span style="color: #6b1c95;">Conjugation</span></strong></p>
Step 2

Immunostaining

<p><strong><span style="color: #6b1c95;">Immunostaining</span></strong></p>
Step 3

Analysis

<p><strong><span style="color: #6b1c95;">Analysis</span></strong></p>

For more detailed information, see the  USER MANUAL or the one page FLYER

Pricing

10 μl FlexiStain™ reagent labels 1 μg biotinylated antibody.

Product Content* Price

FlexiStain™ AlexaFluor 488

500 μl, labels 50 µg biotinylated antibody.

320 €

FlexiStain™ Atto542

500 μl, labels 50 µg biotinylated antibody. A bright cy3 eqivalent.

320 €

FlexiStain™ AlexaFluor 594

500 μl, labels 50 µg biotinylated antibody.

320 €

FlexiStain™ AlexaFluor 647

500 μl, labels 50 µg biotinylated antibody.

320 €

FlexiStain™ HRP (FFPE), for TSA multistaining on paraffin sections

250 μl, labels 25 µg biotinylated antibody.

250 €

FlexiStain™ HRP (Frozen), for TSA multistaining on frozen sections

250 μl, labels 25 µg biotinylated antibody.

250 €

* The Alexa Fluor trademark belongs to LTC and Kromnigon Products contain technology licensed to Kromnigon by LTC under Limited Use Label License (LULL).