SpectraSplit™ upgrades your fluorescence
microscopy by adding more channels.
- Plug-in the SpectraSplit™ filter sets in your
standard light microscope. - Keep using your favorite fluorochromes and
analyze one to three more. - SpectraSplit allows you to analyze Cy3, Alexa594
and Alexa647 without spectral overlap
SpectraSplit™ permits economic use of available light
SpectraSplit™ is a combination of carefully selected light filters to generate more and clearer channels in standard fluorescence microscopy. SpectraSplit™ permits a much more economic use of the available light spectrum by splitting up each of the common channels (green, red, and far-red) into two separate channels.
The channel splits are obtained by the use of condensed filter sets that each occupies a much smaller portion of the light spectrum, without losing the sensitivity for its fluorochrome. In fact, the SpectraSplit™ filter sets have improved signal-to-noise ratios due to its optimized placement of excitation and emission filters that further favor the fluorochrome signal over the background noise.
Products
A wide range of fluorochromes can be visualized with each SpectraSplitTM filter set. The listed price for each
SpectraSplitTM filter set is 1 500 €. However, always ask for an individual quote.
| Options | Explanation | S-Split Filters |
|---|---|---|
| S-Split upgrade (2) Split the red channel into two | Replace the standard red filter set with S-Split 546 and S-Split 594, and keep your standard DAPI + green filter sets. |  |
| S-Split upgrade (3) Split the red channel into two and add a far-red channel | S-Split 647 is designed to block bleedthrough from red fluorochromes |  |
| S-Split upgrade (5) Split the green and the red channel into four and add a far-red channel | Replace the standard green filter set with S-Split 440 and S-Split 488 |  |
| Split DAPI into two | Replace the standard DAPI filter set with S-Split DAPI and S-Split 405 |  |
| Full S-Split setup Add an infra-red channel | S-Split 750 is designed to block bleedthrough from 647 fluorochromes (far-red fluorochromes) |  |