The StreptaClick® Technology

The patented StreptaClick® technology provides monovalent binding of streptavidin to biotinylated antibodies. StreptaClick® is a modified form of tetrameric streptavidin with conserved high binding affinity for biotin. Its monovalent binding properties also prevent antibody aggregation. StreptaClick® can be linked to any biotinylated antibody in a 5-minute mixing reaction, in any volume and without washing steps. Our product portfolio includes StreptaClick® labelled with fluorochromes, enzymes, and site-specifically attached single molecules (oligo, azide, alkyne).

SpectraSplit®7 – Hepta-band optical filter sets for microscopes

Specific separation of 7 fluorescent colors in a multi-stained tissue section has never been easier. The patented SpectraSplit®7 filter sets generate clean, sharp images without the need for spectral unmixing, making it suitable for any downstream application. Simply select among the most popular fluorochromes for each color channel and image without bleed-through.

SpectraSplit®7 was initially developed in 2014. Over the years the hepta-band filter sets have been fine-tuned based on customer feedback to not only fit a wide range of fluorochromes, but also different staining techniques, including Tyramide Signal Amplification and DNA-based multiplexing.

SpectraSplit®7 is compatible with  light sources from CoolLED (pE-800 SB), Lumencor (SPECTRA X light engine) and Excelitas (NOVEM-S).

Introducing StreptaClick® Precision

A groundbreaking version of StreptaClick® developed for site-specific conjugation of a single molecule. StreptaClick® Precision has a unique design with maximum separation of the sites for conjugation and biotin binding. This makes StreptaClick® Precision ideal for attachment of oligonucleotides and other bulky molecules.

StreptaClick® Precision is provided with a single azide or alkyne group for standard click chemistry. It is also available pre-conjugated with a single 14 mer oligo or a single bright fluorochrome (488 and 647).

The combination of single molecule precision and flexibility makes StreptaClick® Precision the ultimate choice for attaching tetrameric streptavidin to biotinylated proteins without aggregation.

Perfectly Suited for oligos

BiotinPure™ Antibody Biotinylation Kit

490 
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Contact us for further information
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SpectraSplit® 7

SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels.  The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Cat no. 7001991-1 Request a Quote

StreptaClick® Color

570 935 
StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer.
  • The StreptaClick® 2-Color Kit contains: StreptaClick® Color 488 and StreptaClick® Color 647. Suitable for multiplex IHC and Flow Cytometry. (Cat no. 50021-1)
  • The StreptaClick® 4-Color Kit contains: StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647. Suitable for multiplex IHC. (Cat no. 50041-1)
Select your StreptaClick® Color Kit:
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