The 7-Color Challenge
Visualizing four colors usually isn’t challenging with conventional bandpass filters. However, when you exceed four colors, the fluorochromes begin to bleed into each other’s channels.
The complete spectrum, from violet to far-red, can only be divided into a certain number of channels before spillover and non-specific signals arise. To achieve a clean signal, each channel must be narrow enough to prevent cross-talk – in other words, maintain specificity – while still capturing as much emission signal as possible.
We have optimized it for you with our patented SpectraSplit® 7, a 7-Channel filter set consisting of 4 bandpass filter sets.
Discover the Power of Seven Colors
Any high-throughput scanning method is enhanced by the use of more colors, as each staining cycle conveys more information. SpectraSplit® 7 is a patented set of bandpass filters that features carefully calculated and tested bandwidths. SpectraSplit® 7 effectively separate seven common classes of fluorochromes without the need for spectral unmixing. The system is compatible with AlexaFluor®dyes, as well as traditional FITC/Cy3/Cy5/Cy7 dyes and many varieties of Opal™ dyes. With its user-friendly design, simply choose one fluorochrome from each class to produce 7-color images free from channel spillover. The data generated requires no spectral unmixing or other types of post-processing.You can proceed directly to analysis with your preferred software.
Fluorochromes compatible with SpectraSplit® 7
|Channel||Traditional dyes||StreptaClick® dyes||ThermoFisher||Biotium||ThermoFisher||Atto-tec||Akoya||Ultivue||Sirigen/BD||Protein
|S-Split Blue (375)||DAPI||AZDye™ 405||Alexa Fluor 405||CF405||Dylight 405||DAPI||DAPI||BV421||Hoecht|
|S-Split Cyan (435)||CF®430||CF430||Atto425||Opal p.480||BV480||cCFP|
|S-Split Green (490)||FITC||AZDye™ 488||Alexa Fluor 488||CF488||DyLight 488||Atto488||Opal 520||Ultivue, FITC||eGFP|
|S-Split Orange (545)||Cy3/TRITC||AZDye™ 555,
Cyanine 3, Atto™ 542
|Alexa Fluor 546||CF555||DyLight 549||Atto542||Opal 570||Ultivue, Cy3||mOrange/
|S-Split Red (590)||Texas Red||AZDye™ 594||Alexa Fluor 594||CF594||DyLight 594||Atto590||Opal 620||mCherry/mRaspberry
|S-Split Far-red (650)||Cy5/Cy5.5||AZDye™ 647,
|Alexa Fluor 647||CF647/680||DyLight 649||Atto647/665||Opal 690||Ultivue, Cy5||miRFP703|
|S-Split Infra-red (740)||Cy7||Alexa Fluor 750||CF750||DyLight 750||Atto740||Opal p.780||Ultivue, Cy7|
*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD
The filter sets are custom made by Chroma Technologies and manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name.
SpectraSplit® 7 maximizes the capacity of any microscope
SpectraSplit® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. SpectraSplit® 7 is designed to work with both standard and scanning fluorescence microscopes and it is compatible with various lighting sources.
SpectraSplit® in the VS200 Research Slide Scanner from Evident
The SpectraSplit® 7 is available as a seven-channel option on the VS200 Research Slide Scanner from Evident. In conjunction with a high-performance microscope, SpectraSplit® 7 enhances your visual capabilities. For more information, please reach out to your Evident sales representative.
We’ve imaged over 2,500 highly-multiplexed slides with the SpectraSplit 7 filters in the past year and the data in every single sample set is crisp and clean. Our users do a large amount of RNAScope staining utilizing the Opal dye set and it’s been a game changer for us to have the SpectraSplit filters that are precisely matched to these dyes. The imaged samples have been a wide range of tissue sections and cultured cells and none of my other microscopes, both filter-based and with spectral detection, match the Opals as well as SpectraSplit 7.
Director, SCOPE Imaging Facility,
UMass Chan Medical School,
Our company Offspring Biosciences supports project teams in the pharma industry with histology-based contract services. Kromnigons SpectraSplit filters have allowed us to significantly expand our capability to perform multiplexed immunofluorescence analyses with excellent separation between the fluorophores. We can highly recommend them.
Managing Director at Offspring