Streptavidin Chemistry

A Novel Form of Streptavidin

The streptavidin-biotin interaction is one of the strongest non-covalent interactions in nature. Given that biotin can be attached to antibodies, other proteins, DNA, vesicles, various surfaces, and more, we have developed StreptaClick®-alkyne and StreptaClick®-azide products with predetermined numbers of alkynes/azides. You can attach your own biomolecules to these, allowing you to adapt StreptaClick® to your assays, target-seeking systems, or production enhancers. The controlled number of alkynes/azides makes it easy for you to find the optimal StreptaClick® product for your needs. Furthermore, neither streptavidin nor biotin is known to cause a significant immune response in most organisms, making it advantageous for use in in vivo studies.

StreptaClick® - Next-generation of streptavidin

StreptaClick®, as the name suggests, is based on the highly recognized streptavidin molecule and its outstanding affinity for biotin. However, StreptaClick® brings a lot more to the table than just its biotin-binding capacity.
Firstly, StreptaClick® is monovalent, which means it can be combined with any biotin-tagged molecule in a solution without forming aggregates. This feature is utilized in our IHC kits, where StreptaClick® with fluorochromes and enzymes are attached to biotinylated antibodies before use in IHC protocol. Despite the monovalency of StreptaClick®, it still possesses the same binding affinity as the naturally occurring tetravalent streptavidin.
Secondly, the StreptaClick® molecule is very suitable as a scaffold to which different types of molecules can be attached. Whether it is a fluorochrome as in StreptaClick®-Color, an enzyme as in StreptaClick®-HRP, or any other biomolecule you choose to attach, the flexibility StreptaClick® offers is truly remarkable.

Create Your Own Extraordinary Molecule

Customize StreptaClick® to fit your specific needs. We provide StreptaClick® pre-equipped with either 1-4 azides or 1-4 alkynes. This allows you to attach 1, 2, 3, or 4 molecules with precision, catering to both small scale labeling and bulk production according to your application needs. Additionally, StreptaClick® retains its biotin-binding capacity and can be easily linked to any biotinylated antibody or protein.

If you’re interested in exploring specific applications with StreptaClick®, please don’t hesitate to contact us.

SpectraSplit® 7

SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels.  The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Request a Quote
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StreptaClick®-Color

330 €
StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick®-color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer. StreptaClick®-color is available in four colors: AZDye™ 488, AZDye™ 594, AZDye™ 647, and Atto™ 542. The AZDyes™ are structurally identical to the Alexa Fluor® dyes, and Atto 542 is a particularly bright dye with a spectral similarity to Cy®3. Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. Select your StreptaClick®-Colors:
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StreptaClick®-HRP Mutliplex IHC Kit

390 € – 850 €
StreptaClick®–HRP antibody labeling reagent and a novel HRP block buffer that efficiently quenches HRP activity between staining cycles. Unlike other buffers, this HRP block buffer does not require boiling between cycles, thus not only reducing the time for each staining cycle but also making it compatible with both FFPE sections and frozen sections, all while preserving tissue morphology. StreptaClick®–HRP is compatible with biotinylated antibodies from any host and on tissues from mice, humans, rats, and other species. Note: While the kit does NOT include TSA Dyes or Signal Amplification buffer, these items are also available for purchase in our online store.
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