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Documentation, videos and publications

DOCUMENTATION

StreptaClick® HRP Mutliplex IHC kit

User Manual
User Manual StreptaClick® HRP

SDS
SDS SE Antibody labeling reagent HRP

SDS EN Antibody labeling reagent HRP

SDS SE HRP block buffer

SDS EN HRP Block Buffer

SDS SE Hydrogen peroxide 3%

SDS EN Hydrogen peroxide 3%

SDS SE Biotin Block Buffer

SDS EN Biotin Block Buffer

SDS SE Block Activator

SDS EN Block Activator

Tyramide dyes

User Manual
Manual Tyramide Dye kit

SDS
SDS EN AZDye™ 405

SDS EN AZDye™ 488

SDS EN AZDye™ 555

SDS EN AZDye™ 594

SDS EN AZDye™ 647

SDS EN CF®430

SDS EN Cyanine 3

SDS EN Cyanine 5

StreptaClick® Color

User Manual
User Manual StreptaClick® Color

SDS
SDS SE Antibody labeling reagent (StreptaClick® Color)

SDS EN Antibody labeling reagent (StreptaClick® Color)

SDS SE Biotin block buffer

SDS EN Biotin block buffer

TSA Signal Amplification Kit

User Manual
Manual Tyramide Dye kit

SDS
SDS EN Tyramide Amplification buccer

BiotinPure™ Antibody Biotinylation Kit

User Manual
Manual BiotinPure™ Antibody Biotinylation kit

StreptaClick® Precision

User Manual
Manual StreptaClick® Precision

SpectraSplit® 7

Folder
SpectraSplit® 7 folder

Protocol for preparation of frozen tissue sections

Protocol
Preparation frozen tissues

VIDEOS

StreptaClick®-Color

Video StreptaClick®-Color

StreptaClick®-Complete TSA Kit

Video StreptaClick®-HRP

StreptaClick®-Precision

Video StreptaClick®-Precision

PUBLICATIONS

StreptaClick®

*Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

*The first publication in which StreptaClick® was used as a tool, making it to the front page of Science Immunology. At the time it was referred to as FlexiStain, the first version of StreptaClick® Color.

SpectraSplit® 7

Bonstingl, L., Zinnegger, M., Sallinger, K. et al. Advanced single-cell and spatial analysis with high-multiplex characterization of circulating tumor cells and tumor tissue in prostate cancer: Unveiling resistance mechanisms with the CoDuCo in situ assay. Biomark Res 12, 140 (2024). https://doi.org/10.1186/s40364-024-00680-z

Gribonika, I. et al. Migratory CD103+CD11b+ cDC2s in Peyer’s patches are critical for gut IgA responses following oral immunization. Mucosal Immunology, Volume 17, Issue 4, 509 – 523, https://doi.org/10.1016/j.mucimm.2024.03.004

Rodin, W., Szeponik, L., Rangelova, T. et al. γδ T cells in human colon adenocarcinomas comprise mainly Vδ1, Vδ2, and Vδ3 cells with distinct phenotype and function. Cancer Immunol Immunother 73, 174 (2024). https://doi.org/10.1007/s00262-024-03758-7

[PREPRINT] Bonstingl, L. et al. Circulating tumor cell characterization and classification by novel combinatorial dual-color (CoDuCo) in situ hybridization and supervised machine learning, bioRxiv 2024.05.08.592946; https://doi.org/10.1101/2024.05.08.592946

Andric, F.; Al-Fairouzi, A.; Wettergren, Y.; Szeponik, L.; Bexe-Lindskog, E.; Cusack, J.C., Jr.; Tumusiime, G.; Quiding-Järbrink, M.; Ljungman, D. Immune Microenvironment in Sporadic Early-Onset versus Average-Onset Colorectal Cancer. Cancers 2023, 15, 1457. https://doi.org/10.3390/cancers15051457

Liang, F.; Nilsson, L.M.; Byvald, F.; Rezapour, A.; Taflin, H.; Nilsson, J.A.; Yrlid, U. A Fraction of CD8+ T Cells from Colorectal Liver Metastases Preferentially Repopulate Autologous Patient-Derived Xenograft Tumors as Tissue-Resident Memory T Cells. Cancers 2022, 14, 2882. https://doi.org/10.3390/cancers14122882

Caër C, Gorreja F, Forsskåhl SK, Brynjolfsson SF, Szeponik L, Magnusson MK, Börjesson LG, Block M, Bexe-Lindskog E, Wick MJ. TREM-1+ Macrophages Define a Pathogenic Cell Subset in the Intestine of Crohn’s Disease Patients. J Crohns Colitis. 2021 Aug 2;15(8):1346-1361. https://doi.org/10.1093/ecco-jcc/jjab022

Glise L, Rutberg M, Håversen L, Levin MC, Levin M, Jeppsson A, Borén J, Fogelstrand P. pH-Dependent Protonation of Histidine Residues Is Critical for Electrostatic Binding of Low-Density Lipoproteins to Human Coronary Arteries. Arterioscler Thromb Vasc Biol. 2022 Aug;42(8):1037-1047. doi: 10.1161/ATVBAHA.122.317868. Epub 2022 Jun 2. PMID: 35652335. https://doi.org/10.1161/ATVBAHA.122.317868

Alsén S, Cervin J, Deng Y, Szeponik L, Wenzel UA, Karlsson J, Cucak H, Livingston M, Bryder D, Lu Q, Johansson-Lindbom B and Yrlid U (2022) Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response. Front. Immunol. 13:813203. https://doi.org/ 10.3389/fimmu.2022.813203

Liang, F.; Rezapour, A.; Szeponik, L.; Alsén, S.; Wettergren, Y.; Bexe Lindskog, E.; Quiding-Järbrink, M.; Yrlid, U. Antigen Presenting Cells from Tumor and Colon of Colorectal Cancer Patients Are Distinct in Activation and Functional Status, but Comparably Responsive to Activated T Cells. Cancers 2021, 13, 5247. https://doi.org/10.3390/cancers13205247

Håversen, L., Sundelin, J.P., Mardinoglu, A. et al. Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice. Sci Rep 8, 16973 (2018). https://doi.org/10.1038/s41598-018-34659-2

Yrlid U, Holm M, Levin M, Alsén S, Lindbom M, Glise L, Bergh N, Borén J, Fogelstrand P. Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density. J Leukoc Biol. 2019 Jan;105(1):195-202. doi: 10.1002/JLB.4A1017-402RR. Epub 2018 Sep 28. PMID: 30265749. https://doi.org/10.1002/JLB.4A1017-402RR

S. Kijani, A. M. Vázquez, M. Levin, J. Borén, P. Fogelstrand. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis, Physiol Rep, 5 (14), 2017, e13334, https://doi.org/10.14814/phy2.13334

** Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P (2015) Filter-Dense Multicolor Microscopy. PLoS ONE 10(3): e0119499. https://doi.org/10.1371/journal.pone.0119499

** The original publication demonstrating what is now our SpectraSplit® bandpass filters for multiplex immunofluorescence microscopy.

POSTERS

* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7

* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.