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BiotinPure™ Antibody Biotinylation Kit
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution.
The antibodies to be biotinylated should be free from other proteins, such as BSA.
The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications.
Cat. no. 9000051-1
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SpectraSplit® 10 (filter sets for 10 color channels)
SpectraSplit® 10 (filter sets for 10 color channels) , developed from our patented SpectraSplit® 7 filter sets, will revolutionize fluorescence microscopy as 10 channels can be directly imaged without spectral unmixing. The channels are distributed over five filter sets and separate the most commonly used fluorochromes in immunofluorescence, in addition to channels anticipated to gain popularity in the near future (see table below). Tissues and other sample types can be immunostained with up to 10 fluorochromes, visualized without bleedthrough. No additional software and no computational spectral unmixing are required.
SpectraSplit® 10 (filter sets for 10 color channels) can easily be installed in both standard and scanning fluorescence microscopes.
Catalog no. 7101991-1 SpectraSplit® 10 (filter sets for 10 color channels)
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SpectraSplit® 7 (filter sets for 7 color channels)
SpectraSplit® 7 (filter sets for 7 color channels) are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence. Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required.
SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources.
Cat no. 7001991-1 SpectraSplit® 7 (filter sets for 7 color channels)
StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology
The StreptaClick® 8-Plex Multiplex IHC Kit provides a rapid and heat-treatment-free Tyramide Signal Amplification (TSA) staining cyclic workflow for frozen and FFPE tissue sections. It is optimized for imaging on the ZEISS Axioscan 7 spatial biology platform, enabling visualization of up to eight colors in a single run. The kit is ideal for researchers in high-throughput laboratories who require a reliable, time-efficient, and flexible multiplex immunohistochemistry workflow.
StreptaClick® Color
StreptaClick® Color is a modified streptavidin that labels biotinylated antibodies with fluorochromes in solution, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer.
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StreptaClick® HRP (heat-free multiplex TSA based IHC)
StreptaClick® HRP Multiplex IHC Kit is an accelerated heat-treatment-free multiplex Tyramide Signal Amplification (TSA) staining solution for up to nine markers (8 antibodies + DAPI). It is suitable for fixed cells, FFPE samples and frozen, OCT embedded tissues. This comprehensive kit is designed for researchers who demand ease of use and flexibility in multiplex immunohistochemistry (IHC).
The kit includes everything needed to label biotinylated primary antibodies with horseradish peroxidase (HRP) enzyme in solution utilizing our modified streptavidin StreptaClick®. As StreptaClick® removes the issue of antibody aggregation through monovalent biotin binding it is particularly suited for labeling primary biotinylated antibodies. The kit also features a novel HRP Quench Buffer that eliminates the need for antibody-stripping by heat-treatment such as boiling between cycles. This feature maintains tissue morphology over multiple staining cycles making it ideal for both frozen and FFPE tissue sections, and higher degrees of multiplexing than comparable solutions.
In combination, these features makes the protocol highly automation-friendly, while results for manual staining of six markers + DAPI are achievable in one day.
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StreptaClick® Precision
StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution.
Click-chemistry ready
StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide).
Pre-conjugated, off-the-shelf
StreptaClick® Precision based, pre-conjugated products are also available. These include StreptaClick® Precision with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm).
Custom conjugation as a service
Please inquire to discuss the feasability of your project.
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