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SpectraSplit® 7

SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels.  The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required.

SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information.

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Description

The SpectraSplit® 7 filter sets are highly efficient in separating the emission signals of seven different fluorochrome classes, with spillover between channels being less than 0.5%. As a result, high-contrast images are generated without the need for spectral unmixing or any other bleed-through corrections.

SpectraSplit® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 7-color.

Fluorochromes
SpectraSplit® 7 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.

Fluorochromes compatible with SpectraSplit® 7

Channel Traditional dyes StreptaClick® dyes ThermoFisher Biotium ThermoFisher Atto-tec Akoya Ultivue Sirigen/BD Protein
expression
fluorophores
S-Split Blue (375) DAPI AZDye™ 405 Alexa Fluor 405 CF405 Dylight 405 DAPI DAPI BV421 Hoecht
S-Split Cyan (435) CF®430 CF430 Atto425 Opal p.480 BV480 cCFP
S-Split Green (490) FITC AZDye™ 488 Alexa Fluor 488 CF488 DyLight 488 Atto488 Opal 520 Ultivue, FITC eGFP
S-Split Orange (545) Cy3/TRITC AZDye™ 555,
Cyanine 3, Atto™ 542		 Alexa Fluor 546 CF555 DyLight 549 Atto542 Opal 570 Ultivue, Cy3 mOrange/
mRFP
S-Split Red (590) Texas Red AZDye™ 594 Alexa Fluor 594 CF594 DyLight 594 Atto590 Opal 620 mCherry/mRaspberry
/mPlum
S-Split Far-red (650) Cy5/Cy5.5 AZDye™ 647,
Cyanine 5		 Alexa Fluor 647 CF647/680 DyLight 649 Atto647/665 Opal 690 Ultivue, Cy5 miRFP703
S-Split Infra-red (740) Cy7 Alexa Fluor 750 CF750 DyLight 750 Atto740 Opal p.780 Ultivue, Cy7

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD

Bandwidth
The approximate mid excitations for the different channels are at 375, 435, 490, 545, 590, 650, and 740nm. However, the exact bandwidths are proprietary information and not publicly disclosed.

Microscopes and light sources
SpectraSplit® 7 is designed to work with both standard and scanning fluorescence microscopes. It is compatible with various lighting sources including pE-800 and pE-4000 from CoolLED, X-Cite NOVEM from Excelitas, and the new SPECTRA X Light Engine from Lumencor.

Hardware
SpectraSplit® 7 contains four custom made filter sets, manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name. Each filter set includes one excitation filter, one emission filter (25 mm in diameter), and one dichroic mirror (25.5 x 36.0 x 1 mm).

Set 1: Triple-band set (375/490/740)
Set 2: Double-band set (435/650)
Set 3: Single-band set (545)
Set 4: Single-band set (590)

Downloads

Folder
SpectraSplit® 7 folder

Additional information

Folder
SpectraSplit® 7 folder

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