SpectraSplit® 7

SpectraSplit^® 7 are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit^® 7 without bleedthrough. No additional software and no computational spectral unmixing are required.

SpectraSplit^® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information.

Cat no. 7001991-1

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Description

The SpectraSplit^® 7 filter sets are highly efficient in separating the emission signals of seven different fluorochrome classes, with spillover between channels being less than 0.5%. As a result, high-contrast images are generated without the need for spectral unmixing or any other bleed-through corrections.

SpectraSplit^® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 7-color images.

Fluorochromes
SpectraSplit^® 7 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.

In addition, our StreptaClick^® reagents for multiplex IHC have been optimized to be compatible with SpectraSplit^® 7 for easy 7-color assay development to make the most of your microscope or scanner equipped with SpectraSplit® 7.

Fluorochromes compatible with SpectraSplit^® 7

Channel	Traditional dyes	StreptaClick® dyes	ThermoFisher	Biotium	ThermoFisher	Atto-tec	Akoya	Ultivue	Sirigen/BD	Protein expression fluorophores
S-Split Blue (375)	DAPI	DAPI, Tyramide 405	Alexa Fluor 405	CF405	Dylight 405		DAPI	DAPI	BV421	Hoecht
S-Split Cyan (435)	CFP	Tyramide CF®430		CF430		Atto425	Opal p.480		BV480	cCFP
S-Split Green (490)	FITC	Tyramide 488, Color 488	Alexa Fluor 488	CF488	DyLight 488	Atto488	Opal 520	Ultivue, FITC		eGFP
S-Split Orange (545)	Cy3/TRITC	Tyramide 555, Color Atto 542	Alexa Fluor 546	CF555	DyLight 549	Atto542	Opal 570	Ultivue, Cy3		mOrange/ mRFP
S-Split Red (590)	Texas Red	Tyramide 594, Color 594	Alexa Fluor 594	CF594	DyLight 594	Atto590	Opal 620			mCherry/mRaspberry /mPlum
S-Split Far-red (650)	Cy5/Cy5.5	Tyramide 647, Color 647	Alexa Fluor 647	CF647/680	DyLight 649	Atto647/665	Opal 690	Ultivue, Cy5		miRFP703
S-Split Infra-red (740)	Cy7	Styramide iFluor 750	Alexa Fluor 750	CF750	DyLight 750	Atto740	Opal p.780	Ultivue, Cy7

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD

Bandwidth
The approximate mid excitations for the different channels are at 375, 435, 490, 545, 590, 650, and 740nm. However, the exact bandwidths are proprietary information and not publicly disclosed.

Microscopes and light sources
SpectraSplit^® 7 is designed to work with both standard and scanning fluorescence microscopes and can be found in scanners from multiple manufacturers including Evident (VS200), TissueGnostics (TissueFAXS CHROMA), and Nikon (Ti2). It is compatible with various lighting sources including pE-800 and pE-4000 from CoolLED, X-Cite NOVEM from Excelitas, and the new SPECTRA X Light Engine from Lumencor.

Hardware
SpectraSplit^® 7 contains four custom made filter sets, manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name. Each filter set includes one excitation filter, one emission filter (25 mm in diameter), and one dichroic mirror (25.5 x 36.0 x 1 mm).

Set 1: Triple-band set (375/490/740)
Set 2: Double-band set (435/650)
Set 3: Single-band set (545)
Set 4: Single-band set (590)

Ordering information

Ordering information