The SpectraSplit® 7 filter sets are highly efficient in separating the emission signals of seven different fluorochrome classes, with spillover between channels being less than 0.5%. As a result, high-contrast images are generated without the need for spectral unmixing or any other bleed-through corrections.
SpectraSplit® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 7-color.
Fluorochromes
SpectraSplit® 7 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.
Fluorochromes compatible with SpectraSplit® 7
*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD
Bandwidth
The approximate mid excitations for the different channels are at 375, 435, 490, 545, 590, 650, and 740nm. However, the exact bandwidths are proprietary information and not publicly disclosed.
Microscopes and light sources
SpectraSplit® 7 is designed to work with both standard and scanning fluorescence microscopes. It is compatible with various lighting sources including pE-800 and pE-4000 from CoolLED, X-Cite NOVEM from Excelitas, and the new SPECTRA X Light Engine from Lumencor.
Hardware
SpectraSplit® 7 contains four custom made filter sets, manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name. Each filter set includes one excitation filter, one emission filter (25 mm in diameter), and one dichroic mirror (25.5 x 36.0 x 1 mm).
Set 1: Triple-band set (375/490/740)
Set 2: Double-band set (435/650)
Set 3: Single-band set (545)
Set 4: Single-band set (590)