The SpectraSplit® 7 filter sets are highly efficient in separating the emission signals of seven different fluorochrome classes, with spillover between channels being less than 0.5%. As a result, high-contrast images are generated without the need for spectral unmixing or any other bleed-through corrections.
SpectraSplit® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 7-color.
SpectraSplit® 7 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.
Fluorochromes compatible with SpectraSplit® 7
|Channel||Traditional dyes||StreptaClick® dyes||ThermoFisher||Biotium||ThermoFisher||Atto-tec||Akoya||Ultivue||Sirigen/BD||Protein
|S-Split Blue (375)||DAPI||AZDye™ 405||Alexa Fluor 405||CF405||Dylight 405||DAPI||DAPI||BV421||Hoecht|
|S-Split Cyan (435)||CF®430||CF430||Atto425||Opal p.480||BV480||cCFP|
|S-Split Green (490)||FITC||AZDye™ 488||Alexa Fluor 488||CF488||DyLight 488||Atto488||Opal 520||Ultivue, FITC||eGFP|
|S-Split Orange (545)||Cy3/TRITC||AZDye™ 555,
Cyanine 3, Atto™ 542
|Alexa Fluor 546||CF555||DyLight 549||Atto542||Opal 570||Ultivue, Cy3||mOrange/
|S-Split Red (590)||Texas Red||AZDye™ 594||Alexa Fluor 594||CF594||DyLight 594||Atto590||Opal 620||mCherry/mRaspberry
|S-Split Far-red (650)||Cy5/Cy5.5||AZDye™ 647,
|Alexa Fluor 647||CF647/680||DyLight 649||Atto647/665||Opal 690||Ultivue, Cy5||miRFP703|
|S-Split Infra-red (740)||Cy7||Alexa Fluor 750||CF750||DyLight 750||Atto740||Opal p.780||Ultivue, Cy7|
*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD
The approximate mid excitations for the different channels are at 375, 435, 490, 545, 590, 650, and 740nm. However, the exact bandwidths are proprietary information and not publicly disclosed.
Microscopes and light sources
SpectraSplit® 7 is designed to work with both standard and scanning fluorescence microscopes. It is compatible with various lighting sources including pE-800 and pE-4000 from CoolLED, X-Cite NOVEM from Excelitas, and the new SPECTRA X Light Engine from Lumencor.
SpectraSplit® 7 contains four custom made filter sets, manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name. Each filter set includes one excitation filter, one emission filter (25 mm in diameter), and one dichroic mirror (25.5 x 36.0 x 1 mm).
Set 1: Triple-band set (375/490/740)
Set 2: Double-band set (435/650)
Set 3: Single-band set (545)
Set 4: Single-band set (590)