Tyramide Dyes and Signal Amplification Buffer 140 € – 150 €
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StreptaClick®-Color

330 €

StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick®-color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer.

StreptaClick®-color is available in four colors: AZDye™ 488, AZDye™ 594, AZDye™ 647, and Atto™ 542. The AZDyes™ are structurally identical to the Alexa Fluor® dyes, and Atto 542 is a particularly bright dye with a spectral similarity to Cy®3.

Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides.

Select your StreptaClick®-Colors:

Atto™ 542
StreptaClick®-Color - Atto™ 542
330 €

In stock

AZDye™ 488
StreptaClick®-Color - AZDye™ 488
330 €

In stock

AZDye™ 594
StreptaClick®-Color - AZDye™ 594
330 €

In stock

AZDye™ 647
StreptaClick®-Color - AZDye™ 647
330 €

In stock

Total items: 0

Total price: 0

Atto™ 542
AZDye™ 488
AZDye™ 594
AZDye™ 647
Clear
Description

The entire multiplex protocol, from labeling to imaging, is completed within an hour. StreptaClick®-antibody conjugates can be combined with DAPI, other directly conjugated primary antibodies or conventional secondary antibodies in the same assay. However, StreptaClick® is not compatible with other streptavidin-based techniques.

Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. The kit also includes a biotin block buffer. This buffer is used to block any remaining free StreptaClick® when multiplexing.

Fluorochrome specifications

Name Absorption max Emission max
AZDye™ 488 – Green 494 nm 517 nm
Atto™ 542 -Yellow 542 nm 562 nm
AZDye™ 594 – Red 590 nm 617 nm
AZDye™ 647 – Far red 649 nm 671 nm

Step-by-Step Multiplex Staining Protocol
Read the comprehensive user manual for detailed instructions.

Should you biotinylate the antibody ‘in house’ using a biotinylation kit, excess free biotin must be removed, potentially via a spin column, before the labeling process.

  1. Add the desired amount of biotinylated antibody to be labeled to a suitably sized tube.
  2. Add the StreptaClick® antibody labeling reagent.
  3. Incubate for 5 minutes
  4. Mix all the antibody-StreptaClick® conjugates in one tube with biotin block buffer.
  5. Apply the mix to your sample and incubate.
  6. Wash, mount, and analyze under a fluorescence microscope.

Storage and Handling
Shipped in room temperature
Store at 4°C, protect from light, do not freeze.
Note: The StreptaClick® antibody labeling reagent contains 0.09% sodium azide.

StreptaClick®-Color Workflow

Unlike any other biotin-streptavidin IHC methods

Attach

Mix each biotinylated antibody with a StreptaClick® color

Mix

Mix all the different StreptaClick®-antibody conjugates in one tube

Multistain

Add all colors simultanously to the slide

Additional information
Color

Atto™ 542, AZDye™ 488, AZDye™ 594, AZDye™ 647
Downloads

User Manual
User Manual StreptaClick®-Color

SDS
SDS SE Antibody labeling reagent (StreptaClick®-Color)

SDS EN Antibody labeling reagent (StreptaClick®-Color)

SDS SE Biotin block buffer

SDS EN Biotin block buffer

Additional information

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Tyramide Dyes and Signal Amplification Buffer

140 € – 150 €
Carefully selected tyramide dyes to provide the brightest panel available on the market, delivering a signal 10-50x brighter than direct conjugates. Although our Tyramide Dyes are optimized and recommended for use with the StreptaClick®-HRP Multiplex IHC kit, they can also be applied in other TSA protocols. Our selection includes 8 optimized tyramide dyes. We suggest a combination of six tyramide dyes along with DAPI. Note that AZDye™ 555 and Cyanine 3 exhibit overlapping signals, as do AZDye™ 647 and Cyanine 5, and thus should not be used simultaneously in the same assay. One vial of tyramide dye is sufficient for 100 stainings (100 ul staining volume). The Signal amplification buffer is sufficient for 250 stainings (100 ul staining volume) Choose your TSA reagents (minimum order 4 TSA dyes):
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StreptaClick®-HRP Mutliplex IHC Kit

390 € – 850 €
StreptaClick®–HRP antibody labeling reagent and a novel HRP block buffer that efficiently quenches HRP activity between staining cycles. Unlike other buffers, this HRP block buffer does not require boiling between cycles, thus not only reducing the time for each staining cycle but also making it compatible with both FFPE sections and frozen sections, all while preserving tissue morphology. StreptaClick®–HRP is compatible with biotinylated antibodies from any host and on tissues from mice, humans, rats, and other species. Note: While the kit does NOT include TSA Dyes or Signal Amplification buffer, these items are also available for purchase in our online store.
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