Ordering information
The StreptaClick® Color 2-plex Kit (Cat no. 50021-1) contains the Labeling Reagents for StreptaClick® Color 488 and StreptaClick® Color 647, along with Biotin Block Buffer. The StreptaClick® 2-Color Kit is suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry.
The StreptaClick® Color 4-plex Kit (Cat no. 50041-1) contains the Labeling Reagents for StreptaClick® Color 488, StreptaClick® Color Atto 542, StreptaClick® Color 594, and StreptaClick® Color 647, along with Biotin Block Buffer. The StreptaClick® Color 4-plex Kit is suitable for multiplex IHC.
Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. The kit also includes a biotin block buffer. This buffer is used to block any remaining free StreptaClick® when multiplexing.
The entire multiplex protocol, from labeling to imaging, is completed within an hour. StreptaClick®-antibody conjugates can be combined with DAPI, other directly conjugated primary antibodies or conventional secondary antibodies in the same assay. However, StreptaClick® is not compatible with other streptavidin-based techniques.
The StreptaClick® Color Complete kits also include our convenient BiotinPure™ Antibody Biotinylation kit in addition to the StreptaClick® Color reagents (2-plex Cat. no. 50022-1 , 4-plex Cat. no. 50042-1)
Fluorochrome specifications
| Name | Absorption max | Emission max |
| StreptaClick® Color 488 – Green | 494 nm | 517 nm |
| StreptaClick® Color Atto 542 -Yellow | 542 nm | 562 nm |
| StreptaClick® Color 594 – Red | 590 nm | 617 nm |
| StreptaClick® Color 647 – Far red | 649 nm | 671 nm |
