StreptaClick® Color

585 959 

StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer.

  • The StreptaClick® Color 2-plex Kit contains: StreptaClick® Color 488 and StreptaClick® Color 647. Suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry. (Cat no. 50021-1)
  • The StreptaClick® Color 4-plex Kit contains: StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647. Suitable for multiplex IHC. (Cat no. 50041-1)

Select your StreptaClick® Color Kit:

StreptaClick® Color 2-plex Kit
StreptaClick® Color - StreptaClick® Color 2-plex Kit
585 

In stock

StreptaClick® Color 4-plex Kit
StreptaClick® Color - StreptaClick® Color 4-plex Kit
959 

In stock

Total items: 0

Total price: 0

StreptaClick® Color 2-plex Kit
StreptaClick® Color 4-plex Kit
Description

The StreptaClick® Color 2-plex Kit contains the Labeling Reagents for StreptaClick® Color 488 and StreptaClick® Color 647, along with Biotin Block Buffer. The StreptaClick® 2-Color Kit is suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry.

The StreptaClick® Color 4-plex Kit contains the Labeling Reagents for StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647, along with Biotin Block Buffer. The StreptaClick® Color 4-plex Kit is suitable for multiplex IHC.

Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides.

The entire multiplex protocol, from labeling to imaging, is completed within an hour. StreptaClick®-antibody conjugates can be combined with DAPI, other directly conjugated primary antibodies or conventional secondary antibodies in the same assay. However, StreptaClick® is not compatible with other streptavidin-based techniques.

Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. The kit also includes a biotin block buffer. This buffer is used to block any remaining free StreptaClick® when multiplexing.

Fluorochrome specifications

Name Absorption max Emission max
StreptaClick® Color 488 – Green 494 nm 517 nm
StreptaClick® Color 542 -Yellow 542 nm 562 nm
StreptaClick® Color 594 – Red 590 nm 617 nm
StreptaClick® Color 647 – Far red 649 nm 671 nm

Step-by-Step Multiplex Staining Protocol
Read the comprehensive user manual for detailed instructions.

Should you biotinylate the antibody ‘in house’ using a biotinylation kit, excess free biotin must be removed, potentially via a spin column, before the labeling process.

  1. Add the desired amount of biotinylated antibody to be labeled to a suitably sized tube.
  2. Add the StreptaClick® antibody labeling reagent.
  3. Incubate for 5 minutes
  4. Mix all the antibody-StreptaClick® conjugates in one tube with biotin block buffer.
  5. Apply the mix to your sample and incubate.
  6. Wash, mount, and analyze under a fluorescence microscope.

Storage and Handling
Shipped in room temperature
Store at 4°C, protect from light, do not freeze.
Note: The StreptaClick® antibody labeling reagent contains 0.09% sodium azide.

StreptaClick®Color Workflow

Unlike any other biotin-streptavidin IHC methods

Attach

Mix each biotinylated antibody with a StreptaClick® Color

Mix

Mix all the different StreptaClick®-antibody conjugates in one tube

Analyze

Add all colors simultanously to the slide for IHC or perform Flow Cytometry

Additional information
Kit

StreptaClick® Color 2-plex Kit

,

StreptaClick® Color 4-plex Kit

Publications

PUBLICATIONS

*Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

*The first publication in which StreptaClick® was used as a tool, making it to the front page of Science Immunology. At the time it was referred to as FlexiStain, the first version of StreptaClick® Color.

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