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Home Products Tyramide Dyes and Signal Amplification Buffer
StreptaClick®-HRP Mutliplex IHC Kit 390 € – 850 €
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StreptaClick®-Color 330 €
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Tyramide Dyes and Signal Amplification Buffer

140 € – 150 €

Carefully selected tyramide dyes to provide the brightest panel available on the market, delivering a signal 10-50x brighter than direct conjugates. Although our Tyramide Dyes are optimized and recommended for use with the StreptaClick®-HRP Multiplex IHC kit, they can also be applied in other TSA protocols.

Our selection includes 8 optimized tyramide dyes. We suggest a combination of six tyramide dyes along with DAPI. Note that AZDye™ 555 and Cyanine 3 exhibit overlapping signals, as do AZDye™ 647 and Cyanine 5, and thus should not be used simultaneously in the same assay.

One vial of tyramide dye is sufficient for 100 stainings (100 ul staining volume).

The Signal amplification buffer is sufficient for 250 stainings (100 ul staining volume)

Choose your TSA reagents (minimum order 4 TSA dyes):

TSA Signal Amplification buffer
Tyramide Dyes and Signal Amplification Buffer - TSA Signal Amplification buffer
140 €

In stock

CF®430
Tyramide Dyes and Signal Amplification Buffer - CF®430
150 €

In stock

Cyanine 3
Tyramide Dyes and Signal Amplification Buffer - Cyanine 3
150 €

In stock

Cyanine 5
Tyramide Dyes and Signal Amplification Buffer - Cyanine 5
150 €

In stock

AZDye™ 405
Tyramide Dyes and Signal Amplification Buffer - AZDye™ 405
150 €

In stock

AZDye™ 488
Tyramide Dyes and Signal Amplification Buffer - AZDye™ 488
150 €

In stock

AZDye™ 555
Tyramide Dyes and Signal Amplification Buffer - AZDye™ 555
150 €

In stock

AZDye™ 594
Tyramide Dyes and Signal Amplification Buffer - AZDye™ 594
150 €

In stock

AZDye™ 647
Tyramide Dyes and Signal Amplification Buffer - AZDye™ 647
150 €

In stock

Total items: 0

Total price: 0

TSA Signal Amplification buffer
CF®430
Cyanine 3
Cyanine 5
AZDye™ 405
AZDye™ 488
AZDye™ 555
AZDye™ 594
AZDye™ 647
Clear
  • Description
  • Additional information
  • Downloads
  • Additional information
Description

Selected Tyramide Dyes and Signal Amplification Buffer for use with the StreptaClick®-HRP Multiplex IHC Kit, available in our online store.

The TSA Signal Amplification buffer includes Tyramide amplification buffer and hydrogen peroxide for HRP activation.

Step-by-Step Multiplex Staining Protocol
Read the comprehensive user manual for detailed instructions.

Storage and Handling

Shipped in room temperature
TSA Dyes: Store at -20°C, Signal Amplification Buffer: Store at 4°C

 

Name Absorption max Emission max
AZDye™ 405 402 nm 424 nm
CF®430 426 nm 498 nm
AZDye™ 488 494 nm 517 nm
AZDye™ 555 555 nm 572 nm
AZDye™ 594 590 nm 617 nm
AZDye™ 647 649 nm 671 nm
Cyanine 3 555 nm 572 nm
Cyanine 5 648 nm 671 nm
Additional information
Color

TSA Signal Amplification buffer, CF®430, Cyanine 3, Cyanine 5, AZDye™ 405, AZDye™ 488, AZDye™ 555, AZDye™ 594, AZDye™ 647

Downloads

User Manual
Manual Tyramide Dye kit

SDS
SDS EN Tyramide Amplification buffer

SDS EN AZDye™ 405

SDS EN AZDye™ 488

SDS EN AZDye™ 555

SDS EN AZDye™ 594

SDS EN AZDye™ 647

SDS EN CF®430

SDS EN Cyanine 3

SDS EN Cyanine 5

Additional information

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StreptaClick®-Color

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StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick®-color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer. StreptaClick®-color is available in four colors: AZDye™ 488, AZDye™ 594, AZDye™ 647, and Atto™ 542. The AZDyes™ are structurally identical to the Alexa Fluor® dyes, and Atto 542 is a particularly bright dye with a spectral similarity to Cy®3. Each color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. Select your StreptaClick®-Colors:
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StreptaClick®-HRP Mutliplex IHC Kit

390 € – 850 €
StreptaClick®–HRP antibody labeling reagent and a novel HRP block buffer that efficiently quenches HRP activity between staining cycles. Unlike other buffers, this HRP block buffer does not require boiling between cycles, thus not only reducing the time for each staining cycle but also making it compatible with both FFPE sections and frozen sections, all while preserving tissue morphology. StreptaClick®–HRP is compatible with biotinylated antibodies from any host and on tissues from mice, humans, rats, and other species. Note: While the kit does NOT include TSA Dyes or Signal Amplification buffer, these items are also available for purchase in our online store.
Select options

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support@kromingon.com

Location

c/o AstraZeneca R&D
Pepparedsleden 1
SE-43183 Mölndal
Sweden

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  • Home
  • Applications
    • Multiplex IHC
    • Streptavidin Chemistry
    • 7-Color Imaging
  • About Us
  • Downloads
  • Products
    • SpectraSplit® 7
    • StreptaClick®-Color
    • StreptaClick®-HRP Mutliplex IHC Kit
    • Tyramide Dyes and Signal Amplification Buffer
  • Shop
  • Contact Us
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