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BiotinPure™ Antibody Biotinylation Kit
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution.
The antibodies to be biotinylated should be free from other proteins, such as BSA.
The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications.
Cat. no. 9000051-1
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SpectraSplit® 7
SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required.
SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information.
Cat no. 7001991-1
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StreptaClick® Color
StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer.
- The StreptaClick® Color 2-plex Kit contains: StreptaClick® Color 488 and StreptaClick® Color 647. Suitable for multiplex IHC and Flow Cytometry. (Cat no. 50021-1)
- The StreptaClick® Color 4-plex Kit contains: StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647. Suitable for multiplex IHC. (Cat no. 50041-1)
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StreptaClick® HRP
Introducing the StreptaClick® HRP Multiplex IHC Kit—your ultimate solution for efficient and high-quality multiplex TSA staining. This comprehensive kit is designed for researchers who demand precision and reliability in immunohistochemistry (IHC). The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP), featuring a novel HRP block buffer that eliminates the need for boiling between cycles, making it ideal for both frozen and FFPE tissue sections.
Select your StreptaClick® HRP Kit:
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StreptaClick® Precision
StreptaClick® Precision is developed for site-specific conjugation with a defined number of attachment groups for click chemistry. Furthermore, StreptaClick® Precision has a unique design with maximum separation of the sites for conjugation and biotin binding. This design makes StreptaClick® Precision ideal for attachment of bulky molecules without affecting the biotin binding affinity and without causing aggregation when mixed with biotinylated proteins.
StreptaClick® Precision is currently provided with a single azide or alkyne group for standard click chemistry. It is also available pre-conjugated with a single 14 mer oligo or a single bright fluorochrome (488 nm or 647 nm).
Internal validation data is available for attaching fluorochromes and phycoerythrin to StreptaClick® Precision. Protocols to attach enzymes and oligonucleotides are currently in development, as well as a StreptaClick® Precision with a DBCO group for copper free click chemistry. External partners have successfully attached other molecules, including lipid nanoparticles.
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