SpectraSplit 7

Direct 7-color visualization of multistained tissues (no spectral unmixing)
SpectraSplit 7 is unique filter sets for direct visualizing of 7-color multiplex IHC. No spectral unmixing is needed, just load samples and acquire images. The filter sets are compatible with standard and scanning fluorescence microscopes of various brands.

Easy to use – no single stained slides, no time spent on post processing
SpectraSplit 7 separates each fluorochrome directly by the filter sets without computer-based post-processing. You do not have to include single stained sections and no time-consuming excecution of spectral unmixing is required. Hence, SpectraSplit 7 speeds up the image acquisition. In addition, its simplicity greatly increases access to all users as no extra training is needed to go from three to seven colors.

Outstanding fluorochrome separation and image quality
SpectraSplit 7 has less than 0.5% spillover between any of the channels, which generates an efficient fluorochrome separation. The image quality is outstanding due to high fluorochrome specificity over background noise. This is of extra importance when the staining is weak and for tissues with high autofluorescence.

Multiband filter sets 
SpectraSplit 7 is configured in four filter sets – one triple band, one double band, and two single band filter sets. 

Light sources 
SpectraSplit 7 can be run with most multi-channel light sources, including pE-800 and pE-4000 from CoolLED, X-Cite NOVEM from Excelitas, and Spectra III Light Engine from Lumencor. 

Compatible with a large number of common fluorochromes
SpectraSplit 7 is fully compatible with a wide range of common fluorochromes, including AlexaFluor® dyes, the classic FITC/Cy3/Cy5/Cy7 dyes, and the Opal™ Polaris 7-color manual IHC detection kit. Hence, you have a large variety of fluorochrome options when setting up a 7-color IHC (Table below).

Customer feedback

“We’ve imaged over 2,500 highly-multiplexed slides with the SpectraSplit 7 filters in the past year and the data in every single sample set is crisp and clean. Our users do a large amount of RNAScope staining utilizing the Opal dye set and it’s been a game changer for us to have the SpectraSplit filters that are precisely matched to these dyes. The imaged samples have been a wide range of tissue sections and cultured cells and none of my other microscopes, both filter-based and with spectral detection, match the Opals as well as SpectraSplit 7.”

Christina Baer, Director, SCOPE Imaging Facility, UMass Chan Medical School, Worcester MA

“Our company Offspring Biosciences supports project teams in the pharma industry with histology-based contract services. Kromnigons SpectraSplit filters have allowed us to significantly expand our capability to perform multiplexed immunofluorescence analyses with excellent separation between the fluorophores. We can highly recommend them.”

– Anders Dahlstrand, Managing Director at Offspring Bioscience –


Please contact info@kromnigon for quotes and information about adding extra channels to your microscope.

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