StreptaClick® Technology

A Novel Form of Streptavidin

The streptavidin-biotin interaction is one of the strongest non-covalent interactions in nature. Given that biotin can be attached to antibodies, other proteins, oligos and DNA, vesicles, various surfaces, and more, we have developed the StreptaClick® molecule. StreptaClick® provides the specificity of monovalent streptavidin without sacrificing its binding affinity to biotin. Furthermore, neither streptavidin nor biotin is known to cause a significant immune response in most organisms, making it advantageous for use in in vivo studies.

StreptaClick® — The next generation of streptavidin

StreptaClick®, as the name suggests, is based on the highly recognized streptavidin molecule. Despite having been engineered it still conserves native steptavidin’s outstanding affinity for biotin. However, StreptaClick® brings a lot more to the table than just its biotin-binding capacity.

Firstly, StreptaClick® is monovalent, which means it can be combined with any biotin-tagged molecule in a solution without forming aggregates. This feature is utilized in our immunohistochemistry (IHC) kits, where StreptaClick® with fluorochromes or enzymes are attached to biotinylated antibodies before use in a IHC protocol. Despite the induced monovalency of StreptaClick®, it still possesses the same binding affinity as the naturally occurring tetravalent streptavidin.

Secondly, the StreptaClick® molecule is very suitable as a scaffold to which different types of molecules can be attached. Whether it is a fluorochrome as in StreptaClick®-Color, an enzyme as in StreptaClick®-HRP, or any other biomolecule you choose to attach as in StreptaClick®-Precision, the flexibility StreptaClick® offers is truly remarkable.

Create Your Own Extraordinary Molecule

Tailor StreptaClick® to match your precise requirements. Our range includes StreptaClick® Precision variants featuring a predetermined number of alkyne or azide groups (ranging from 1 to 4*), specifically designed for click chemistry. This enables the precise attachment of 1, 2, 3, or 4* molecules, catering to both small-scale labeling and large-scale production, perfectly aligning with your application needs. You have the flexibility to attach your biomolecules, adapting StreptaClick® Precision to complement your assays, target-seeking systems, or production enhancements. The controlled number of alkynes or azides* simplifies the process of selecting the optimal StreptaClick® product to your specific needs. Furthermore, StreptaClick® Precision maintains its remarkable biotin-binding capability and effortlessly connects to any biotinylated antibody or protein.

*1 available, 2-4 in development

Should you wish to delve into specific applications using StreptaClick®, please don’t hesitate to get in touch with us.

Applications