StreptaClick® Technology -
avoid antibody aggregation
A Novel Form of Streptavidin
The streptavidin-biotin interaction is one of the strongest non-covalent interactions in nature. Given that biotin can be attached to antibodies, other proteins, oligos and DNA, vesicles, various surfaces, and more, we have developed an improved streptavidin-based molecule – the StreptaClick® molecule. StreptaClick® provides the specificity of monovalent streptavidin without sacrificing its binding affinity to biotin. Furthermore, neither streptavidin nor biotin is known to cause a significant immune response in most organisms, making it advantageous for use in in vivo studies.
StreptaClick® — The next generation of streptavidin
StreptaClick®, as the name suggests, is based on the highly recognized streptavidin molecule. Despite having been engineered it still conserves native steptavidin’s outstanding affinity for biotin. However, StreptaClick® brings a lot more to the table than just its biotin-binding capacity.
Firstly, StreptaClick® is monovalent, which means it can be combined with any biotin-tagged molecule in a solution without forming aggregates. This feature is utilized in our immunohistochemistry (IHC) kits, where StreptaClick® with fluorochromes or enzymes are attached to biotinylated antibodies before use in a IHC protocol. Despite the induced monovalency of StreptaClick®, it still possesses the same binding affinity as the naturally occurring tetravalent streptavidin.
Secondly, the StreptaClick® molecule is very suitable as a scaffold to which different types of molecules can be attached. Whether it is a fluorochrome as in StreptaClick® Color, an enzyme as in StreptaClick® HRP, or any other biomolecule you choose to attach via click chemistry as in StreptaClick® Precision, the flexibility StreptaClick® offers is truly remarkable.
Create Your Own Extraordinary Molecule
Tailor StreptaClick® to match your precise requirements. Our product range includes StreptaClick® Precision – 1 Azide, specifically designed for Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. This enables the precise attachment of exactly one molecule, catering to applications requiring a 1:1 ratio of reporter to binder. The azide reactive group provides flexibility to attach your biomolecules via different chemistries, adapting StreptaClick® Precision to complement your assays, target-seeking systems, or production enhancements. Furthermore, StreptaClick® Precision maintains its remarkable biotin-binding capability and effortlessly connects to any biotinylated antibody or protein via its novel cis-divalent design, featuring two clustered biotin binding sites. The clustered dual binding sites ensure biotin binding is not blocked by bulky molecules such as oligonucleotides.
Possible applications include antibody-oligonucleotide conjugate based methods such as immuno-PCR, immuno-RCA, Proximity Ligation Assay, Proximity Extension Assay as well as others such as prototyping antibody-drug conjugates or targeting antibodies conjugated to delivery systems such as lipid nanoparticles or extracelluar vesicles.
Should you require a greater ratio of reporters per binder we are currently developing StreptaClick® Precision versions with exactly three or seven reactive groups.
If you wish to delve into specific applications using StreptaClick®, please do not hesitate to get in touch with us.
