SpectraSplit® 7 - Multicolor Imaging

Visualizing four colors usually isn’t challenging with conventional bandpass filters. However, when you exceed four colors, the fluorochromes begin to bleed into each other’s channels.
The complete spectrum, from violet to far-red, can only be divided into a certain number of channels before spillover and non-specific signals arise. To achieve a clean signal, each channel must be narrow enough to prevent cross-talk – in other words, maintain specificity – while still capturing as much emission signal as possible.

We have optimized it for you with our patented SpectraSplit® 7, a 7-Channel filter set consisting of 4 bandpass filter sets.

Discover the Power of Seven Colors

Any high-throughput scanning method is enhanced by the use of more colors, as each staining cycle conveys more information. SpectraSplit® 7 is a patented set of bandpass filters that features carefully calculated and tested bandwidths. SpectraSplit^® 7 effectively separate seven common classes of fluorochromes without the need for spectral unmixing. The system is compatible with AlexaFluor^®dyes, as well as traditional FITC/Cy3/Cy5/Cy7 dyes and many varieties of Opal™ dyes. With its user-friendly design, simply choose one fluorochrome from each class to produce 7-color images free from channel spillover. The data generated requires no spectral unmixing or other types of post-processing.You can proceed directly to analysis with your preferred software.

Fluorochromes compatible with SpectraSplit^® 7

Channel	Traditional dyes	StreptaClick® dyes	ThermoFisher	Biotium	ThermoFisher	Atto-tec	Akoya	Ultivue	Sirigen/BD	Protein expression fluorophores
S-Split Blue (375)	DAPI	AZDye™ 405	Alexa Fluor 405	CF405	Dylight 405		DAPI	DAPI	BV421	Hoecht
S-Split Cyan (435)		CF®430		CF430		Atto425	Opal p.480		BV480	cCFP
S-Split Green (490)	FITC	AZDye™ 488	Alexa Fluor 488	CF488	DyLight 488	Atto488	Opal 520	Ultivue, FITC		eGFP
S-Split Orange (545)	Cy3/TRITC	AZDye™ 555, Cyanine 3, Atto™ 542	Alexa Fluor 546	CF555	DyLight 549	Atto542	Opal 570	Ultivue, Cy3		mOrange/ mRFP
S-Split Red (590)	Texas Red	AZDye™ 594	Alexa Fluor 594	CF594	DyLight 594	Atto590	Opal 620			mCherry/mRaspberry /mPlum
S-Split Far-red (650)	Cy5/Cy5.5	AZDye™ 647, Cyanine 5	Alexa Fluor 647	CF647/680	DyLight 649	Atto647/665	Opal 690	Ultivue, Cy5		miRFP703
S-Split Infra-red (740)	Cy7		Alexa Fluor 750	CF750	DyLight 750	Atto740	Opal p.780	Ultivue, Cy7

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brillialt Violet dyes are trademarks of Sirigen/BD

Quality Filters

The filter sets are custom made by Chroma Technologies and manufactured to the high product standards of Chroma Technologies. SpectraSplit® 7 is a patented technology, exclusive to our brand. These filter sets are not available under any other name.

SpectraSplit^® 7 maximizes the capacity of any microscope

SpectraSplit^® 7 immediately empowers your microscope with seven independent color channels that each generates crystal clear images. SpectraSplit^® 7 is designed to work with both standard and scanning fluorescence microscopes and it is compatible with various lighting sources.

SpectraSplit^® in the VS200 Research Slide Scanner from Evident

The SpectraSplit^® 7 is available as a seven-channel option on the VS200 Research Slide Scanner from Evident. In conjunction with a high-performance microscope, SpectraSplit^® 7 enhances your visual capabilities. For more information, please reach out to your Evident sales representative.

We’ve imaged over 2,500 highly-multiplexed slides with the SpectraSplit 7 filters in the past year and the data in every single sample set is crisp and clean. Our users do a large amount of RNAScope staining utilizing the Opal dye set and it’s been a game changer for us to have the SpectraSplit filters that are precisely matched to these dyes. The imaged samples have been a wide range of tissue sections and cultured cells and none of my other microscopes, both filter-based and with spectral detection, match the Opals as well as SpectraSplit 7.

Christina Baer,
Director, SCOPE Imaging Facility,
UMass Chan Medical School,
Worcester MA

Our company Offspring Biosciences supports project teams in the pharma industry with histology-based contract services. Kromnigons SpectraSplit filters have allowed us to significantly expand our capability to perform multiplexed immunofluorescence analyses with excellent separation between the fluorophores. We can highly recommend them.

Anders Dahlstrand,
Managing Director at Offspring
Bioscience

BiotinPure™ Antibody Biotinylation Kit

503 €

The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Contact us for further information

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SpectraSplit® 7

SpectraSplit^® 7 are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit^® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit^® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Cat no. 7001991-1 Request a Quote

StreptaClick® Color

585 € – 959 €

StreptaClick® Color labels antibodies with fluorochromes, mimicking directly conjugated antibodies. StreptaClick^®Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The click reaction is highly efficient and can be carried out at any volume, concentration, or buffer.

The StreptaClick® Color 2-plex Kit contains: StreptaClick® Color 488 and StreptaClick® Color 647. Suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry. (Cat no. 50021-1)
The StreptaClick® Color 4-plex Kit contains: StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647. Suitable for multiplex IHC. (Cat no. 50041-1)

Select your StreptaClick® Color Kit:

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SpectraSplit® 7 - Multicolor Imaging