StreptaClick® Precision for Click Chemistry
StreptaClick® Precision — Monovalently Binding Streptavidin for Click Chemistry
Customize StreptaClick® to match your precise requirements. Our product range includes the novel StreptaClick® Precision – 1 Azide molecule, specifically designed for Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. This enables the precise attachment of exactly one molecule, catering to applications requiring a 1:1 ratio of reporter to binder. The azide reactive group provides flexibility to attach your biomolecules via different chemistries, adapting StreptaClick® Precision to complement your assays, target-seeking systems, or production enhancements. Furthermore, StreptaClick® Precision maintains its remarkable biotin-binding capability and effortlessly connects to any biotinylated antibody or protein via its two clustered biotin binding sites in cis-position. The dual binding sites ensure biotin binding is not blocked by bulky molecules such as oligonucleotides.
Possible applications include antibody-oligonucleotide conjugate based methods such as immuno-PCR, immuno-RCA, Proximity Ligation Assay, Proximity Extension Assay as well as others such as prototyping antibody-drug conjugates or targeting antibodies conjugated to delivery systems such as lipid nanoparticles or extracelluar vesicles.
Should you require a greater ratio of reporters per binder we are currently developing StreptaClick® Precision versions with exactly three or seven reactive groups.
If you’re interested in exploring specific applications with StreptaClick® Precision, please do not hesitate to contact us.
StreptaClick® Precision - a cis-divalent streptavidin primed for Click Chemistry
Introducing StreptaClick® Precision – 1 Azide, your ultimate tool for precise protein labeling. Our engineered cis-divalent yet monovalently binding streptavidin features exactly one azide functional group strategically attached with maximum distance from the dual biotin binding sites to minimize the risk of bulky reporter molecules obscuring biotin-streptavidin binding reaction. This offers unparalleled control in your labeling process.
StreptaClick® Precision differs from our previous StreptaClick® Color and StreptaClick® HRP molecules in that it has been redesigned with dual biotin binding sites clustered in cis-position. This design still exhibits monovalent binding properties to antibodies while conserving its native binding affinity to biotin, ensuring unmatched specificity in molecular interactions. In early experiments we observed that monovalent StreptaClick® could have its biotin binding obscured by bulky molecules such as oligonucleotides, a problem that has now been solved with StreptaClick® Precision.
StreptaClick® Precision Workflow
Click Chemistry
Revolutionize your scientific endeavors with our cutting-edge range of click chemistry products. Engineered to streamline and amplify your research, our line offers versatile solutions tailored to meet diverse needs.
From customizable StreptaClick® variants, equipped with precisely controlled azide groups for seamless molecule attachment, to a comprehensive selection of click-compatible reagents and tools, our products empower you to drive innovation in drug development, biomolecule labeling, and beyond. Experience efficiency and precision in every reaction, allowing you to unleash the full potential of click chemistry in your laboratory. Join the forefront of scientific advancement with our click chemistry solutions and transform your experiments into groundbreaking discoveries.
How it works
With StreptaClick® Precision, the labeling process becomes seamless.
Easily attach the azide labeled StreptaClick® Precision molecule to your reporter molecule of choice via click chemistry. The azide functionalization enables either Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry, ensuring accurate and specific labeling in many different types of experiments. We primarily recommend SPAAC with DBCO- functionalized reporters, but alkyne functionalized reporters also work well.
Achieve high conjugation efficiency
Conjugating reporters to StreptaClick® Precision via CuAAC or SPAAC click chemistry typically results in 90-100% labeling efficiency with only 1.3X excess of reporter to StreptaClick® molecules.
Gel photo comparing StreptaClick® – oligo conjugations to controls
- StreptaClick® Precision – Azide + 20 bp oligo – DBCO
- StreptaClick® Precision – Azide + 20 bp oligo – DBCO
- Control: StreptaClick® Precision – Azide
- StreptaClick® Precision – DBCO + 43 bp oligo – azide
- StreptaClick® Precision – DBCO + 41 bp oligo – azide
- Control: StreptaClick® Precision – DBCO
- Marker
- StreptaClick® Precision – DBCO + 41 bp oligo – azide
Attach StreptaClick® Precision conjugate to any biotinylated antibody or surface
After the click chemistry reaction, add the newly formed StreptaClick® Precision construct to your biotinylated protein or surface of interest for near immediate conjugation. We also have a convenient biotinylation kit available for purchase, BiotinPureTM, if you lack access to biotinylated antibodies.
This innovation grants you exquisite control over labeling positions and ratios, enabling detailed exploration of protein behaviors, interactions, and localization.
The image below shows an example of a fresh frozen mouse spleen tissue in which StreptaClick® Precision – 1 Oligo bound to an anti B220 biotinylated antibody from Biolegend interacts with B220 proteins on B-lymphocytes. The interaction is visualized via immunofluorescence imaging by hybridization of an antisense oligo with a 647nm fluorochrome to StreptaClick® Precision – 1 Oligo. Other possible readouts for antibody-oligonucleotide conjugate based methods include q-PCR and DNA sequencing.
Monovalently Binding Streptavidin for Click Chemistry
From advanced bioconjugation techniques for high-resolution imaging in super-resolution microscopy to facilitating targeted drug delivery systems, StreptaClick® Precision’s versatility knows no bounds. Streamline protein purification processes, enhance protein detection assays, and empower oligonucleotide research with precise modifications and labeling. Its adaptability extends further, allowing the development of custom click chemistry-based assays for biomarker detection and molecular interaction studies.
With StreptaClick® Precision, delve into a multitude of applications requiring easy, versatile and tightly controlled conjugation, empowering scientists and researchers to explore, innovate, and elevate their discoveries to unprecedented heights across diverse scientific domains.
BiotinPure™ Antibody Biotinylation Kit
SpectraSplit® 7
StreptaClick® Color
- The StreptaClick® Color 2-plex Kit contains: StreptaClick® Color 488 and StreptaClick® Color 647. Suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry. (Cat no. 50021-1)
- The StreptaClick® Color 4-plex Kit contains: StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647. Suitable for multiplex IHC. (Cat no. 50041-1)
StreptaClick® HRP
