StreptaClick® HRP
Price range: 400 € through 1734 €
StreptaClick® HRP Multiplex IHC Kit is an accelerated heat-treatment-free multiplex Tyramide Signal Amplification (TSA) staining solution with imaging for up to six colors for both frozen and FFPE samples in one day. This comprehensive kit is designed for researchers who demand ease of use, flexibility, precision and reliability in immunohistochemistry (IHC).
The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP) enzyme in solution utilizing our modified streptavidin StreptaClick®. As StreptaClick® removes the issue of antibody aggregation through monovalent biotin binding it is particularly suited for labeling primary biotinylated antibodies. The kit also features a novel HRP block buffer that eliminates the need for antibody-stripping by heat-treatment such as boiling between cycles. This feature maintains tissue morphology over multiple staining cycles making it ideal for both frozen and FFPE tissue sections, and higher degrees of multiplexing than comparable solutions.
In combination, these features makes the protocol automation friendly and results for six color + DAPI experiments are achievable in one day.
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your StreptaClick® HRP Kit:
StreptaClick® HRP Workflow
How it works
Biotinylated antibodies pre-mixed with StreptaClick® operate similarly to directly conjugated antibodies, thereby eliminating the need for secondary antibodies. The StreptaClick® labeling reaction is extremely efficient, permitting the labeling of any antibody volume, even down to microliters. This is particularly beneficial when setting up new experiments.
Quick Results, Less Hands On Time
With StreptaClick® HRP you can complete a 7-color Tyramide Signal Amplification (TSA) multiplex IHC within a single day (DAPI + 6 biotinylated antibodies). Each biotinylated antibody is labeled with HRP by the fast StreptaClick® reaction, and the kit includes an HRP block buffer that efficiently quenches HRP activity between staining cycles. This avoids the slow, inconvenient and harsh boiling step to strip off antibodies required by other solutions between staining cycles. Added benefits of the HRP block buffer include preserved tissue morphology, higher attainable protein biomarker multiplexing levels and a protocol that is automation-friendly.
Seven-Color TSA method for frozen sections from mice, humans, rats, and other species, and FFPE
The StreptaClick® HRP solution is a must for any manual or automated TSA staining. The kit comes with a HRP quenching buffer that eliminates the need for boiling, which not only accelerates each color cycle but also makes the kit highly beneficial for frozen tissue sections. The quenching buffer effectively silences the HRP signal from the previous round while preserving the morphology of the frozen tissue. Hence, the StreptaClick® HRP kit provides a rapid method for multiplex TSA IHC on both FFPE and frozen sections, with antibodies from any host, and on tissues from mice, humans, rats, and other species.
Rapid manual or automated protocol
Automation is also facilitated by the rapid protocol and unique features. The use of primary antibodies and our HRP quenching buffer makes it especially amenable for automated staining. External partners have developed a protocol on the Leica Biosystems BOND RX IHC staining system, and protocols for other systems are in development.
Imaging
Fluorochrome specifications
| Name | Absorption max | Emission max |
| Tyramide CF®430 | 426 nm | 498 nm |
| Tyramide 488 | 494 nm | 517 nm |
| Tyramide 555 | 555 nm | 572 nm |
| Tyramide 594 | 590 nm | 617 nm |
| Tyramide 647 | 648 nm | 671 nm |
| Styramide iFluor 750 | 757 nm | 779 nm |
The fluorochromes we provide are carefully chosen to align with most common filter sets.
However, the inclusion of more fluorochromes in your immunostainings adds complexity. For reliable imaging of tissues stained with up to seven colors (7-plex IHC) without requiring spectral unmixing, we offer a patented combination of filter sets— SpectraSplit® 7. SpectraSplit® 7 accommodates all the channels in the chart and performs excellently with AZDyes and a variety of commercial dyes, such as AlexaFluor® dyes, FITC, TRITC, Cy3, Cy5, Cy7 dyes, and many of the OpalTM dyes. Learn more
| Channel | Traditional dyes | StreptaClick® dyes |
| S-Split Blue (375) | DAPI | DAPI |
| S-Split Cyan (435) | CFP | Tyramide CF®430 |
| S-Split Green (490) | FITC | Tyramide 488 |
| S-Split Orange (545) | Cy3/TRITC | Tyramide 555 |
| S-Split Red (590) | Texas Red | Tyramide 594 |
| S-Split Far-red (650) | Cy5/Cy5.5 | Tyramide 647 |
| S-Split Infra-red (740) | Cy7 | Styramide iFluor® 750 |
Frequently Asked Questions
Yes, StreptaClick® HRP heat-free TSA is easy to use and well-suited for those new to the field. The heat-free protocol considerably reduces the optimization time required to build a multiplex antibody panel.
Through its TSA and HRP block system, the need for secondary antibodies is eliminated. This improves reproducibility and streamlines the workflow compared to indirect immunofluorescence (IF), while preserving target epitopes and tissue morphology.
Up to 5-7 antibodies can be applied within a single day depending on the required incubation time of each antibody, significantly reducing the total experiment time. On average one staining cycle takes 1.5 hours.
Yes, StreptaClick® HRP heat-free TSA has been developed and validated for use with biotinylated primary antibodies. Biotinylated primary antibodies can be purchased from a number of vendors including our listed partners, either off the shelf or as custom orders. Alternatively, carrier free antibodies (ie no carrier protein such as BSA, and ideally free from sodium azide) can easily be biotinylated with our BiotinPure™ Antibody Biotinylation Kit. BiotinPure™ is an easy and complete solution only requiring a benchtop centrifuge.
| StreptaClick HRP | HRP Quench Buffer kit (250 reactions), StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions), StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions), StreptaClick® HRP Multiplex IHC kit (250 reactions), StreptaClick® HRP Multiplex IHC kit (90 reactions), Styramide iFluor® 750 (100-200 reactions), Tyramide Dye 3-color kit (250 reactions), Tyramide Dye 5-color kit (250 reactions), Tyramide Dye 5-color kit (500 reactions) |
|---|
Ordering information
| Cat no. | Product |
| 6008182-1 | StreptaClick® HRP Multiplex IHC kit (90 reactions) |
| 6008181-1 | StreptaClick® HRP Multiplex IHC kit (250 reactions) |
| 60031-1 | Tyramide 3-color Dye kit (300-600 reactions) |
| 60051-1 | Tyramide 5-color Dye kit (500-1000 reactions) |
| 6007501-1 | Styramide iFluor® 750 kit (100 - 200 reactions) |
| 63B8181-1 | StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit |
| 65B8181-1 | StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit |
Please contact us to inquire about larger pack sizes and custom kit configurations.
StreptaClick® HRP Multiplex IHC kits
The kits contain StreptaClick® HRP antibody labeling reagent for labeling of biotinylated antibodies, and a novel horseradish peroxidase (HRP) block buffer. The staining protocol does not require heat treatment such as boiling between cycles, and is ideal for direct labeling of primary antibodies for higher degrees of multiplexing compared to working with secondary antibodies.
Tyramide Dye kits
- Tyramide 3-color Dye kit: includes Tyramide 488, Tyramide 555, Tyramide 647 and Tyramide Amplification Buffer Plus.
- Tyramide 5-color Dye kit: includes Tyramide CF®430, Tyramide 488, Tyramide 555, Tyramide 594, Tyramide 647, and Tyramide Amplification Buffer Plus.
- Styramide iFluor® 750 kit: includes Styramide iFluor® 750, and Tyramide Amplification Buffer Plus. Purchase separately to add a sixth dye at the far end of the spectrum.
StreptaClick® HRP Complete TSA IHC kits
The complete kits contain all components necessary for 3-color or 5-color Tyramide Signal Amplification (TSA) multiplex staining. Just add primary antibodies.
- Components included: StreptaClick® HRP Multiplex IHC kit (250 reactions) + Tyramide Dye kit (250 reactions) + BiotinPure™ Antibody Biotinylation Kit
RESOURCES
FLYER
StreptaClick® HRP flyer
USER MANUAL
User Manual StreptaClick® HRP
USER MANUAL (Tyramide Dyes)
User Manual Tyramide Dyes
SDS
SDS EN SCHRP KIT - Antibody labeling reagent (StreptaClick HRP)_2.1_60810
SDS EN SCHRP KIT - 3% H2O2_2.1_60806
SDS EN SCHRP KIT - Biotin block buffer_2.1_60804
SDS EN SCHRP KIT - Block activator_v2
SDS EN SCHRP KIT - HRP block buffer_2.1_60805
SDS EN Tyramide dyes_1.0_76444
SDS Biotium Tyramide Amplification Buffer PLUS
PUBLICATIONS
2025
POSTERS
* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7
* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.
PUBLICATIONS
Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!
You may also like…
BiotinPure™ Antibody Biotinylation Kit
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your BiotinPure™ Kit:Related products
SpectraSplit® 10 filter sets (COMING SOON)
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
SpectraSplit® 7
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology
StreptaClick® Color
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
