StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology
The StreptaClick® 8-Plex Multiplex IHC Kit provides a rapid and heat-treatment-free Tyramide Signal Amplification (TSA) staining cyclic workflow for frozen and FFPE tissue sections. It is optimized for imaging on the ZEISS Axioscan 7 spatial biology platform, enabling visualization of up to eight colors in a single run. The kit is ideal for researchers in high-throughput laboratories who require a reliable, time-efficient, and flexible multiplex immunohistochemistry workflow.
Next-Generation TSA Staining – Powered by StreptaClick® HRP
The workflow is based on the StreptaClick® technology, which uses a modified streptavidin to link reporter molecules to primary biotinylated antibodies in solution.
The StreptaClick® HRP workflow transforms TSA staining into a heat-free, faster, and simpler process for both manual and automated applications, offering four key advantages over conventional protocols:
- Significantly shortened TSA workflow. The StreptaClick® technology eliminates the use of secondary antibodies, accelerating the protocol while maintaining full flexibility in primary antibody species selection.
- A heat-free workflow that preserves epitopes and tissue morphology. The proprietary HRP blocking buffer is specifically optimized to ensure efficient HRP inactivation while maintaining optimal tissue integrity – even on frozen tissue sections.
- Improved resistance to tyrosine depletion. The heat-free workflow does not strip off antibodies. As a result, new tyrosine residues are incorporated into the tissue with each antibody, making the method less susceptible to tyrosine depletion.
- Easy multiplex panel setup. By reducing epitope exhaustion and tyrosine depletion, the staining conditions remain more consistent across cycles. Consequently, the order of antibodies does not need to be optimized, which saves considerable time when establishing new panels.
Staining frozen tissue sections
Staining human FFPE tissue sections
StreptaClick® 8-plex Workflow
Labeling your primary antibodies with StreptaClick® HRP
Biotinylated antibodies pre-mixed with StreptaClick® HRP function in the same way as directly conjugated antibodies, eliminating the need for secondary antibodies. The StreptaClick® labeling reaction is highly efficient, allowing you to label any antibody volume — even as little as a few microliters. This makes it especially useful for testing new experimental setups or labs often changing antibody panels for different projects.
Multiplex immunofluorescence protocol
- Apply the first antibody to the tissue
- Develop color with TSA reagents
- Quench HRP activity with HRP block buffer
- Repeat the cycle with the next antibody until all have been developed
Each cycle takes approximately 1 hour to complete. Tyramide Signal Amplification with our carefully chosen tyramide dyes and amplification buffer provides 5-20X signal amplification over directly labeled antibodies.
Automatically scan the slide in the ZEISS Axioscan 7 spatial biology once all tyramide dyes have been developed.
Fast results, less hands on time
With StreptaClick® 8-plex, you can complete a 7-color Tyramide Signal Amplification (TSA) multiplex IHC assay in a single day (DAPI + seven biotinylated antibodies). Each biotinylated antibody is rapidly labeled with HRP through the StreptaClick® HRP reaction. The kit also includes a specialized HRP block buffer that effectively quenches HRP activity between staining cycles, eliminating the need for the slow, harsh boiling step typically required to strip antibodies between cycles. One cycle thereby only takes 1 hour, compared to 2.5 hours for traditional TSA workflows.
This HRP block buffer not only simplifies the workflow but also preserves tissue morphology, enables higher levels of protein biomarker multiplexing, and ensures compatibility with automated systems.
Rapid manual or automated protocol
The streamlined protocol and innovative features of the StreptaClick® 8-plex kit make it well-suited for both manual and automated staining. By using primary antibodies along with the included HRP quenching buffer, automation becomes straightforward and efficient.
Available protocols:
- Leica Biosystems BOND RX IHC staining system
- Roche Ventana Discovery Plus (IN DEVELOPMENT)
In combination with imaging on the ZEISS Axioscan 7 spatial biology it represents an ideal workflow solution for high-throughput laboratories, whether they are a CRO or an academic or pharma core facility.
Automated imaging with the ZEISS Axioscan 7 spatial biology
The ZEISS Axioscan 7 spatial biology is the newest iteration of the system, ideal for high-throughput laboratories as 100 slides can simultaneously be loaded and rapidly imaged in 8 color channels.
Fully Automated System
- Simple to operate
- Automated Tissue Detection, Focusing, and Scanning
- Reliable and Reproducible Data
Multiplex Imaging Flexibility
- Customizable light source and filters
- Not limited to proprietary reagent chemistries
- Motorized condenser for polarization, brightfield, and TIE
24/7 operation
- Load and unload slides while you image
- Change slide priority on the fly
- Fully automated imaging: walk away
Streamlined 8-plex IHC for High-Throughput Laboratories
The StreptaClick® 8-plex immunofluorescence kit streamlines high-throughput multiplex IHC by eliminating secondary antibodies and enabling rapid, efficient labeling even at microliter volumes. Its integrated HRP block buffer removes harsh boiling steps, preserving tissue morphology and allowing same-day completion of up to 7-color Tyramide Signal Amplification (TSA) assays.
In addition, the heat-free multiplex TSA protocol potentially saves months of development time for establishing new antibody panels as the cycle order at which the antibodies are added is no longer critical. Compatible with FFPE and frozen tissues from multiple species, and easily automated on systems like the Leica Biosystems BOND RX, StreptaClick® 8-plex delivers faster turnaround, higher multiplexing capability, and reliable results across diverse research and translational projects when combined with imaging on the ZEISS Axioscan 7 spatial biology.
Ordering information
Please reach out to your local ZEISS Microscopy sales representative or distributor to learn more.
RESOURCES
FLYER
StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology flyer
POSTERS
* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7
* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.
PUBLICATIONS
Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!
Related products
BiotinPure™ Antibody Biotinylation Kit
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution.
The antibodies to be biotinylated should be free from other proteins, such as BSA.
The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications.
Cat. no. 9000051-1
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your BiotinPure™ Kit:
SpectraSplit® 10 filter sets
SpectraSplit® 10, developed from our patented SpectraSplit® 7 filter sets, will revolutionize fluorescence microscopy as 10 channels can be directly imaged without spectral unmixing. The channels are distributed over five filter sets and separate the most commonly used fluorochromes in immunofluorescence, in addition to channels anticipated to gain popularity in the near future (see table below). Tissues and other sample types can be immunostained with up to 10 fluorochromes, visualized without bleedthrough. No additional software and no computational spectral unmixing are required.
SpectraSplit® 10 can easily be installed in both standard and scanning fluorescence microscopes.
Request a quote or information
SpectraSplit® 7
SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels. The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required.
SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information.
Cat no. 7001991-1
Request a quote or information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
StreptaClick® Color
StreptaClick® Color is a modified streptavidin that labels biotinylated antibodies with fluorochromes in solution, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer.
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your StreptaClick® Color Kit:
StreptaClick® HRP
StreptaClick® HRP Multiplex IHC Kit is an accelerated heat-treatment-free multiplex Tyramide Signal Amplification (TSA) staining solution for up to nine markers (8 antibodies + DAPI) for fixed cells, FFPE samples and frozen tissues. This comprehensive kit is designed for researchers who demand ease of use and flexibility in multiplex immunohistochemistry (IHC).
The kit includes everything needed to label biotinylated primary antibodies with horseradish peroxidase (HRP) enzyme in solution utilizing our modified streptavidin StreptaClick®. As StreptaClick® removes the issue of antibody aggregation through monovalent biotin binding it is particularly suited for labeling primary biotinylated antibodies. The kit also features a novel HRP Quench Buffer that eliminates the need for antibody-stripping by heat-treatment such as boiling between cycles. This feature maintains tissue morphology over multiple staining cycles making it ideal for both frozen and FFPE tissue sections, and higher degrees of multiplexing than comparable solutions.
In combination, these features makes the protocol highly automation-friendly, while results for manual staining of six markers + DAPI are achievable in one day.
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your StreptaClick® HRP Kit:
StreptaClick® Precision
StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution.
Click-chemistry ready
StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide).
Pre-conjugated, off-the-shelf
StreptaClick® Precision based, pre-conjugated products are also available. These include StreptaClick® Precision with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm).
Custom conjugation as a service
Please inquire to discuss the feasability of your project.
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your StreptaClick® Precision Kit:
