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StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology

The StreptaClick® 8-Plex Multiplex IHC Kit provides a rapid and heat-treatment-free Tyramide Signal Amplification (TSA) staining cyclic workflow for frozen and FFPE tissue sections. It is optimized for imaging on the ZEISS Axioscan 7 spatial biology platform, enabling visualization of up to eight colors in a single run. The kit is ideal for researchers in high-throughput laboratories who require a reliable, time-efficient, and flexible multiplex immunohistochemistry workflow.

Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.

We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).

Description

Next-Generation TSA Staining – Powered by StreptaClick® HRP

 

The workflow is based on the StreptaClick® technology, which uses a modified streptavidin to link reporter molecules to primary biotinylated antibodies in solution.

The StreptaClick® HRP workflow transforms TSA staining into a heat-free, faster, and simpler process for both manual and automated applications, offering four key advantages over conventional protocols:

  1. Significantly shortened TSA workflow. The StreptaClick® technology eliminates the use of secondary antibodies, accelerating the protocol while maintaining full flexibility in primary antibody species selection.
  2. A heat-free workflow that preserves epitopes and tissue morphology. The proprietary HRP blocking buffer is specifically optimized to ensure efficient HRP inactivation while maintaining optimal tissue integrity – even on frozen tissue sections.
  3. Improved resistance to tyrosine depletion. The heat-free workflow does not strip off antibodies. As a result, new tyrosine residues are incorporated into the tissue with each antibody, making the method less susceptible to tyrosine depletion.
  4. Easy multiplex panel setup. By reducing epitope exhaustion and tyrosine depletion, the staining conditions remain more consistent across cycles. Consequently, the order of antibodies does not need to be optimized, which saves considerable time when establishing new panels.

Staining frozen tissue sections

Staining human FFPE tissue sections

StreptaClick® 8-plex Workflow

Labeling your primary antibodies with StreptaClick® HRP

Biotinylated antibodies pre-mixed with StreptaClick® HRP function in the same way as directly conjugated antibodies, eliminating the need for secondary antibodies. The StreptaClick® labeling reaction is highly efficient, allowing you to label any antibody volume — even as little as a few microliters. This makes it especially useful for testing new experimental setups or labs often changing antibody panels for different projects.

Multiplex immunofluorescence protocol

  1. Apply the first antibody to the tissue
  2. Develop color with TSA reagents
  3. Quench HRP activity with HRP block buffer
  4. Repeat the cycle with the next antibody until all have been developed

Each cycle takes approximately 1 hour to complete. Tyramide Signal Amplification with our carefully chosen tyramide dyes and amplification buffer provides 5-20X signal amplification over directly labeled antibodies.

Automatically scan the slide in the ZEISS Axioscan 7 spatial biology once all tyramide dyes have been developed.

Fast results, less hands on time

With StreptaClick® 8-plex, you can complete a 7-color Tyramide Signal Amplification (TSA) multiplex IHC assay in a single day (DAPI + seven biotinylated antibodies). Each biotinylated antibody is rapidly labeled with HRP through the StreptaClick® HRP reaction. The kit also includes a specialized HRP block buffer that effectively quenches HRP activity between staining cycles, eliminating the need for the slow, harsh boiling step typically required to strip antibodies between cycles. One cycle thereby only takes 1 hour, compared to 2.5 hours for traditional TSA workflows.

This HRP block buffer not only simplifies the workflow but also preserves tissue morphology, enables higher levels of protein biomarker multiplexing, and ensures compatibility with automated systems.

Rapid manual or automated protocol

The streamlined protocol and innovative features of the StreptaClick® 8-plex kit make it well-suited for both manual and automated staining. By using primary antibodies along with the included HRP quenching buffer, automation becomes straightforward and efficient.

Available protocols:

  • Leica Biosystems BOND RX IHC staining system
  • Roche Ventana Discovery Plus (IN DEVELOPMENT)

In combination with imaging on the ZEISS Axioscan 7 spatial biology it represents an ideal workflow solution for high-throughput laboratories, whether they are a CRO or an academic or pharma core facility.

Automated imaging with the ZEISS Axioscan 7 spatial biology

The ZEISS Axioscan 7 spatial biology is the newest iteration of the system, ideal for high-throughput laboratories as 100 slides can simultaneously be loaded and rapidly imaged in 8 color channels.

Fully Automated System

  • Simple to operate
  • Automated Tissue Detection, Focusing, and Scanning
  • Reliable and Reproducible Data

Multiplex Imaging Flexibility

  • Customizable light source and filters
  • Not limited to proprietary reagent chemistries
  • Motorized condenser for polarization, brightfield, and TIE

24/7 operation

  • Load and unload slides while you image
  • Change slide priority on the fly
  • Fully automated imaging: walk away

Learn more about the system from ZEISS Microscopy

Streamlined 8-plex IHC for High-Throughput Laboratories

The StreptaClick® 8-plex immunofluorescence kit streamlines high-throughput multiplex IHC by eliminating secondary antibodies and enabling rapid, efficient labeling even at microliter volumes. Its integrated HRP block buffer removes harsh boiling steps, preserving tissue morphology and allowing same-day completion of up to 7-color Tyramide Signal Amplification (TSA) assays. Compatible with FFPE and frozen tissues from multiple species, and easily automated on systems like the Leica Biosystems BOND RX, it delivers faster turnaround, higher multiplexing capability, and reliable results across diverse research and translational projects when combined with imaging on the ZEISS Axioscan 7 spatial biology.

Ordering information

Ordering information

Order the StreptaClick® 4-plex starter kit or StreptaClick® 8-plex starter kit to receive all the reagents necessary to label biotinylated antibodies with StreptaClick® HRP and perform multiplex tyramide signal amplification, including Kromnigon's unique HRP blocking buffer.

Alternatively, combine StreptaClick®, reagent kits with tyramide dyes of choice (minimum three individual tyramide dyes per order, DAPI optional) to develop the multiplex panels of your choice.

Cat no. Product Description
6007181-1 StreptaClick®, reagent kit (250 manual/75 automated rxn) includes StreptaClick® HRP labeling reagent, HRP blocking reagents, and Tyramide amplification buffer. Sufficient for 250 manual/75 automated rxn (Leica BOND RX).
6007185-1 StreptaClick®, reagent kit (1000 manual/300 automated rxn) includes StreptaClick® HRP labeling reagent, HRP blocking reagents, and Tyramide amplification buffer. Sufficient for 1000 manual/300 automated rxn (Leica BOND RX).
6004301-1 Tyramide CF® 430 includes Tyramide CF®430 dye). Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
6004881-1 Tyramide 488 includes Tyramide 488 dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
6005551-1 Tyramide 555 includes Tyramide 555 dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
6005941-1 Tyramide 594 includes Tyramide 594 dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
6006471-1 Tyramide 647 includes Tyramide 647 dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
6007501-1 Styramide iFluor® 750 includes Styramide iFluor® 750 dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
600490LS-1 Tyramide ATTO 490LS includes Tyramide ATTO 490LS dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
600DAP1-1 DAPI includes DAPI nuclear staining dye. Sufficient for 100-200 manual/120 automated rxn (Leica BOND RX).
604000D-1 StreptaClick® 4-plex, dye kit (DAPI + 3 tyramide dyes) includes Tyramide dyes 488, 555, 647, and DAPI. Each dye sufficient for 100-200 manual/120 automated rxn.
608000D-1 StreptaClick® 8-plex, dye kit (DAPI + 7 tyramide dyes) includes Tyramide dyes CF®430, 488, 555, 594, 647, ATTO 490LS, Styramide iFluor®750, and DAPI. Each dye sufficient for 100-200 manual/120 automated rxn.
604025D-1 StreptaClick® 4-plex starter kit (250 manual/75 automated rxn) includes one StreptaClick® reagent kit (250 manual/75 automated rxn) and one StreptaClick® 4-plex, dye kit (DAPI + 3 tyramide dyes. Each dye sufficient for 100-200 manual/120 automated rxn).
604100D-1 StreptaClick® 4-plex starter kit (1000 manual/300 automated rxn) includes one StreptaClick® reagent kit (1000 manual/300 automated rxn) and one StreptaClick® 4-plex, dye kit (DAPI + 3 tyramide dyes. Each dye sufficient for 100-200 manual/120 automated rxn).
608025D-1 StreptaClick® 8-plex starter kit (250 manual/75 automated rxn) includes one StreptaClick® reagent kit (250 manual/75 automated rxn) and one StreptaClick® 8-plex, dye kit (DAPI + 3 tyramide dyes. Each dye sufficient for 100-200 manual/120 automated rxn).
608100D-1 StreptaClick® 8-plex starter kit (1000 manual/300 automated rxn) includes one StreptaClick® reagent kit (1000 rxn) and one StreptaClick® 8-plex, dye kit (DAPI + 3 tyramide dyes. Each dye sufficient for 100-200 manual/120 automated rxn).
9000051-1 BiotinPure™ Antibody Biotinylation Kit (5 rxn) convenient spin column based antibody biotinylation kit

Please contact us to inquire about larger pack sizes and custom kit configurations.

Fluorochrome specifications

We have chosen an optimized selection of tyramide dyes with high signal intensity and great compatibility.

Name Absorption max Emission max
Tyramide CF®430 426 nm 498 nm
Tyramide 488 494 nm 517 nm
Tyramide ATTO 490LS 496nm 661nm
Tyramide 555 555 nm 572 nm
Tyramide 594 590 nm 617 nm
Tyramide 647 648 nm 671 nm
Styramide iFluor® 750 757 nm 779 nm

 

Multiplexing table - understand the effect of multiplexing and manual vs automated workflows

RXN manual workflow RXN Automated workflow (LEICA BOND RX)
StreptaClick®, reagent kit (250 rxn) X Colors 1 1 3 5 6 7
Reactions at X colors 250 75 25 15 12 10
No. Of 10 slide runs 25 7 2 1 1 1
No. Of 20 slide runs 12 3 1 0 0 0
No. Of 30 slide runs 8 2 0 0 0 0
StreptaClick®, reagent kit (1000 rxn) X Colors 1 1 3 5 6 7
Reactions at X colors 1000 300 100 60 50 40
No. Of 10 slide runs 100 30 10 6 5 4
No. Of 20 slide runs 50 15 5 3 2 2
No. Of 30 slide runs 33 10 3 2 1 1
Tyramide dyes X Colors 1 1 3 5 6 7
Reactions at X colors 200 120 120 120 120 120
No. Of 10 slide runs 20 12 12 12 12 12
No. Of 20 slide runs 10 6 6 6 6 6
No. Of 30 slide runs 6 4 4 4 4 4

 

Resources

RESOURCES

FLYER
StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology flyer

 

POSTERS
* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7

* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.

PUBLICATIONS

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Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
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Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
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Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
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Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® HRP Kit:
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Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Precision Kit:
Select options This product has multiple variants. The options may be chosen on the product page