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StreptaClick® Color 585 € – 1159 €
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StreptaClick® HRP

400 € – 1734 €

StreptaClick® HRP Multiplex IHC Kit is your ultimate solution for efficient and high-quality multiplex Tyramide Signal Amplification (TSA) staining. This comprehensive kit is designed for researchers who demand precision and reliability in immunohistochemistry (IHC).

The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP) utilizing our modified streptavidin StreptaClick® that removes the issue of antibody aggregation through monovalent binding. The kit also features a novel HRP block buffer that eliminates the need for boiling between cycles, making it ideal for both frozen and FFPE tissue sections.

Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.

We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.

At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.

NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).

Select your StreptaClick® HRP Kit:

StreptaClick® HRP Multiplex IHC kit (90 reactions)
StreptaClick® HRP - StreptaClick® HRP Multiplex IHC kit (90 reactions)
400 €

In stock

StreptaClick® HRP Multiplex IHC kit (250 reactions)
StreptaClick® HRP - StreptaClick® HRP Multiplex IHC kit (250 reactions)
872 €

In stock

Tyramide Dye 3-color kit (250 reactions)
StreptaClick® HRP - Tyramide Dye 3-color kit (250 reactions)
605 €

In stock

Tyramide Dye 5-color kit (250 reactions)
StreptaClick® HRP - Tyramide Dye 5-color kit (250 reactions)
811 €

In stock

Tyramide Dye 5-color kit (500 reactions)
StreptaClick® HRP - Tyramide Dye 5-color kit (500 reactions)
1016 €

In stock

iFluor 750 Styramide (250 reactions)
StreptaClick® HRP - iFluor 750 Styramide (250 reactions)
522 €

In stock

StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)
StreptaClick® HRP - StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)
1529 €

In stock

StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)
StreptaClick® HRP - StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)
1734 €

In stock

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Description

Ordering information

StreptaClick® HRP kits

  • StreptaClick® HRP Multiplex IHC kit (90 reactions). (Cat. no. 6008182-1)
  • StreptaClick® HRP Multiplex IHC kit (250 reactions). (Cat. no. 6008181-1)

The kit contains StreptaClick® HRP antibody labeling reagent and a novel horseradish peroxidase (HRP) block buffer. The staining protocol does not require boiling between cycles, and is compatible with biotinylated antibodies from any host species.

Tyramide Dye kits

  • Tyramide 3-color Dye kit includes: Tyramide 488, Tyramide 555, Tyramide 647 and Tyramide Amplification Buffer Plus. (Cat. no.60031-1)
  • Tyramide 5-color Dye kit includes: Tyramide CF®430, Tyramide 488, Tyramide 555, Tyramide 594, Tyramide 647, and Tyramide Amplification Buffer Plus. (Cat. no. 60051-1)
  • iFluor 750 Styramide, purchase separately to add a sixth dye at the far end of the spectrum.  (Cat. no. 6007501-1)

StreptaClick® HRP Complete TSA IHC kits 

The complete kits contain all components necessary for 3-plex (Cat. no. 63B8181-1) or 5-plex (Cat. no. 65B8181-1) Tyramide Signal Amplification (TSA) multiplex staining. Just add antibodies.

  • Components included: StreptaClick® HRP Multiplex IHC kit (250 reactions) +  Tyramide Dye kit (250 reactions) + BiotinPure™ Antibody Biotinylation Kit (3 or 5 reactions)
  • Tyramide Dyes in the 3-color kit: Tyramide 488, Tyramide 555, Tyramide 647
  • Tyramide Dyes in the 5-color kit: Tyramide CF®430, Tyramide 488, Tyramide 555, Tyramide 594, Tyramide 647

StreptaClick® HRP Workflow

StreptaClick HRP multiplex TSA protocol

Step-by-Step Multiplex Staining Protocol
Read the comprehensive user manual for detailed instructions.

Biotinylate your antibodies with the BiotinPure™ Antibody labeling kit

  1. Mix each biotinylated antibody with StreptaClick® HRP labeling reagent
  2. Incubate at least 10 minutes
  3. Add biotin block buffer to quench unreacted StreptaClick reagent
  4. Prepare the slide for immunostaining according to standard protocol. Avidin/biotin block is not needed.
  5. Block endogenous peroxidases with the provided HRP block buffer.
  6. Apply the first HRP-labeled antibody to your tissue sections and incubate 20-60 minutes at RT. Wash x3 in PBS.
  7. Dilute tyramide fluorochrome in tyramide amplification buffer and apply to your samples. Incubate 12 minutes at RT and wash x3 in water.
  8. Apply activated HRP block buffer and incubate for 12 minutes at RT.
  9. Wash x2 in water and x1 in PBS
  10. Repeat steps 6-9 for all HRP labeled antibodies.
  11. Wash, mount, and analyze under a fluorescence microscope.

Storage and Handling
Shipped in room temperature
Store at 4°C.

Additional information
HRP kits

StreptaClick® HRP Multiplex IHC kit (90 reactions)

,

StreptaClick® HRP Multiplex IHC kit (250 reactions)

,

Tyramide Dye 3-color kit (250 reactions)

,

Tyramide Dye 5-color kit (250 reactions)

,

Tyramide Dye 5-color kit (500 reactions)

,

iFluor 750 Styramide (250 reactions)

,

StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)

,

StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)
Downloads

FLYER
StreptaClick® HRP flyer

USER MANUAL
User Manual StreptaClick® HRP

USER MANUAL (Tyramide Dyes)
User Manual Tyramide Dyes

SDS
SDS SE Antibody labeling reagent HRP

SDS EN Antibody labeling reagent HRP

SDS SE Biotin block buffer HRP

SDS EN Biotin block buffer HRP

SDS SE Hydrogen peroxide 3%

SDS EN Hydrogen peroxide 3%

Publications

POSTERS
* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7

* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.

Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!

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BiotinPure™ Antibody Biotinylation Kit

503 €
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your BiotinPure™ Kit:
Select options This product has multiple variants. The options may be chosen on the product page

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    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
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    StreptaClick® Color

    585 € – 1159 €
    StreptaClick® Color is a modified streptavidin that labels antibodies with fluorochromes, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer.
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    • The StreptaClick® Color Complete kits also include our convenient BiotinPure™ Antibody Biotinylation kit in addition to the StreptaClick® Color reagents (2-plex Cat. no. 50022-1 , 4-plex Cat. no. 50042-1)
    Order or request further information

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
    Select options This product has multiple variants. The options may be chosen on the product page

    StreptaClick® Precision

    400 € – 3694 €
    StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution. StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide). Pre-conjugated StreptaClick® Precision products are also available. These include StreptaClick® Precision pre-conjugated with a single 14mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm). Order or request further information  

    Request a quote or order

    Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

    Webshop

    Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Precision Kit:
    Select options This product has multiple variants. The options may be chosen on the product page