StreptaClick® HRP

400 1734 

StreptaClick® HRP Multiplex IHC Kit is your ultimate solution for efficient and high-quality multiplex Tyramide Signal Amplification (TSA) staining. This comprehensive kit is designed for researchers who demand precision and reliability in immunohistochemistry (IHC).

The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP) utilizing our modified streptavidin StreptaClick® that removes the issue of antibody aggregation through monovalent binding. The kit also features a novel HRP block buffer that eliminates the need for boiling between cycles, making it ideal for both frozen and FFPE tissue sections.

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Select your StreptaClick® HRP Kit:

StreptaClick® HRP Multiplex IHC kit (90 reactions)
StreptaClick® HRP - StreptaClick® HRP Multiplex IHC kit (90 reactions)
400 

In stock

StreptaClick® HRP Multiplex IHC kit (250 reactions)
StreptaClick® HRP - StreptaClick® HRP Multiplex IHC kit (250 reactions)
872 

In stock

Tyramide Dye 3-color kit (250 reactions)
StreptaClick® HRP - Tyramide Dye 3-color kit (250 reactions)
605 

In stock

Tyramide Dye 5-color kit (250 reactions)
StreptaClick® HRP - Tyramide Dye 5-color kit (250 reactions)
811 

In stock

Tyramide Dye 5-color kit (500 reactions)
StreptaClick® HRP - Tyramide Dye 5-color kit (500 reactions)
1016 

In stock

iFluor 750 Styramide (250 reactions)
StreptaClick® HRP - iFluor 750 Styramide (250 reactions)
522 

In stock

StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)
StreptaClick® HRP - StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)
1529 

In stock

StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)
StreptaClick® HRP - StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)
1734 

In stock

Total items: 0

Total price: 0

Description

Ordering information

StreptaClick® HRP kits

  • StreptaClick® HRP Multiplex IHC kit (90 reactions). (Cat. no. 6008182-1)
  • StreptaClick® HRP Multiplex IHC kit (250 reactions). (Cat. no. 6008181-1)

The kit contains StreptaClick® HRP antibody labeling reagent and a novel horseradish peroxidase (HRP) block buffer. The staining protocol does not require boiling between cycles, and is compatible with biotinylated antibodies from any host species.

Tyramide Dye kits

  • Tyramide 3-color Dye kit includes: Tyramide 488, Tyramide 555, Tyramide 647 and Tyramide Amplification Buffer Plus. (Cat. no.60031-1)
  • Tyramide 5-color Dye kit includes: Tyramide CF®430, Tyramide 488, Tyramide 555, Tyramide 594, Tyramide 647, and Tyramide Amplification Buffer Plus. (Cat. no. 60051-1)
  • iFluor 750 Styramide, purchase separately to add a sixth dye at the far end of the spectrum.  (Cat. no. 6007501-1)

StreptaClick® HRP Complete TSA IHC kits 

The complete kits contain all components necessary for 3-plex (Cat. no. 63B8181-1) or 5-plex (Cat. no. 65B8181-1) Tyramide Signal Amplification (TSA) multiplex staining. Just add antibodies.

  • Components included: StreptaClick® HRP Multiplex IHC kit (250 reactions) +  Tyramide Dye kit (250 reactions) + BiotinPure™ Antibody Biotinylation Kit (3 or 5 reactions)
  • Tyramide Dyes in the 3-color kit: Tyramide 488, Tyramide 555, Tyramide 647
  • Tyramide Dyes in the 5-color kit: Tyramide CF®430, Tyramide 488, Tyramide 555, Tyramide 594, Tyramide 647

StreptaClick® HRP Workflow

StreptaClick HRP multiplex TSA protocol

Step-by-Step Multiplex Staining Protocol
Read the comprehensive user manual for detailed instructions.

Biotinylate your antibodies with the BiotinPure™ Antibody labeling kit

  1. Mix each biotinylated antibody with StreptaClick® HRP labeling reagent
  2. Incubate at least 10 minutes
  3. Add biotin block buffer to quench unreacted StreptaClick reagent
  4. Prepare the slide for immunostaining according to standard protocol. Avidin/biotin block is not needed.
  5. Block endogenous peroxidases with the provided HRP block buffer.
  6. Apply the first HRP-labeled antibody to your tissue sections and incubate 20-60 minutes at RT. Wash x3 in PBS.
  7. Dilute tyramide fluorochrome in tyramide amplification buffer and apply to your samples. Incubate 12 minutes at RT and wash x3 in water.
  8. Apply activated HRP block buffer and incubate for 12 minutes at RT.
  9. Wash x2 in water and x1 in PBS
  10. Repeat steps 6-9 for all HRP labeled antibodies.
  11. Wash, mount, and analyze under a fluorescence microscope.

Storage and Handling
Shipped in room temperature
Store at 4°C.

Additional information
HRP kits

StreptaClick® HRP Multiplex IHC kit (90 reactions)

,

StreptaClick® HRP Multiplex IHC kit (250 reactions)

,

Tyramide Dye 3-color kit (250 reactions)

,

Tyramide Dye 5-color kit (250 reactions)

,

Tyramide Dye 5-color kit (500 reactions)

,

iFluor 750 Styramide (250 reactions)

,

StreptaClick® HRP Complete 3-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (3 reactions)

,

StreptaClick® HRP Complete 5-color TSA IHC kit (250 reactions). HRP kit + Tyramide Dye kit + BiotinPure kit (5 reactions)

Publications

POSTERS
* Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7

* Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.

Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!