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SpectraSplit® 10 filter sets (COMING SOON)

SpectraSplit® 10, developed from our patented SpectraSplit® 7 filter sets, will revolutionize fluorescence microscopy as 10 channels can be directly imaged without spectral unmixing.  The channels are distributed over five filter sets and separate the most commonly used fluorochromes in immunofluorescence, in addition to channels anticipated to gain popularity in the near future (see table below). Tissues and other sample types can be immunostained with up to 10 fluorochromes, visualized without bleedthrough. No additional software and no computational spectral unmixing are required.

SpectraSplit® 10 can easily be installed in both standard and scanning fluorescence microscopes.

COMING SOON

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Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.

We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD.

NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).

Description

SpectraSplit® 10 - pushing the boundaries of optical physics

The SpectraSplit® 10 filter sets are highly efficient in separating the emission signals of ten different fluorochrome classes, with spillover between channels being less than 0.5% for the majority of the channels. Consequently, high-contrast images for ten distinct channels are generated without the need for spectral unmixing or any other corrections – a unique accomplishment.

SpectraSplit® 10 immediately and uniquely empowers your microscope with ten independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 10-color images.

Fluorochromes
SpectraSplit® 10 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.

In addition, our own StreptaClick® reagents for multiplex IHC have been optimized to be compatible with SpectraSplit® 7 for easy 7-color assay development to make the most of your microscope or scanner equipped with SpectraSplit® 7. We will increase the number of StreptaClick® reagents for the launch of SpectraSplit® 10 to allow users to fully unleash the multiplexing power of the filter sets.

Fluorochromes compatible with SpectraSplit® 10

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Aluora, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brilliant Violet dyes are trademarks of Sirigen/BD

Filter channels
The channels will be at 365, 405, 435, 490, 545, 590, 650, 740 and 850 nm. However, the exact bandwidths are proprietary information and not publicly disclosed.

Microscopes and light sources
SpectraSplit® 10 is designed to work with both standard and scanning fluorescence microscopes.

Contact us if you want to know more or discuss testing SpectraSplit® 10 ahead of launch as one of our early access customers.

Ordering information

Ordering information

Cat no. Product
TBD SpectraSplit® 10

COMING SOON. Please contact us to inquire about custom filter configurations and to learn more ahead of product launch.

Resources

RESOURCES - FOR SpectraSplit® 7

FOLDER
SpectraSplit® 7 folder

PUBLICATIONS

[PREPRINT] Sebastian Bessler et.al., Single-cell mass spectrometry imaging combined with immunofluorescence reveals neutrophil heterogeneity in inflammation, bioRxiv preprint doi: https://doi.org/10.1101/2025.08.19.671021

Luis Enrique Sastré-Velásquez et.al., Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers, Journal of Biological Engineering (2025) 19:69, https://doi.org/10.1186/s13036-025-00539-3

[PREPRINT] Alexander Potthoff et. Al., Spatial biology using single-cell mass spectrometryimaging and integrated microscopy, Research Square Preprint, https://doi.org/10.21203/rs.3.rs-6751055/v1

[PREPRINT] Sukanya Iyer, Katelyn Daman, Yehui Sun et. al., Efficient Cas9 nuclease-based editing in skeletal muscle via lipid nanoparticle delivery., bioRxiv preprint doi: https://doi.org/10.1101/2025.05.12.653518

Elena Garre, Sara Rhost, Anna Gustafsson, Louis Szeponik, Thais Fenz Araujo, Marianne Quiding-Järbrink, Khalil Helou, Anders Ståhlberg & Göran Landberg, Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity, Communications Biology | ( 2025) 8:621, https://doi.org/10.1038/s42003-025-08054-3

[PREPRINT] Fike AJ, Bricker KN, Gonzalez MV, et. al. IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints. bioRxiv [Preprint]. 2025 Feb 8:2025.02.04.636277. doi: 10.1101/2025.02.04.636277

Bonstingl, L., Zinnegger, M., Sallinger, K. et al. Advanced single-cell and spatial analysis with high-multiplex characterization of circulating tumor cells and tumor tissue in prostate cancer: Unveiling resistance mechanisms with the CoDuCo in situ assay. Biomark Res 12, 140 (2024). https://doi.org/10.1186/s40364-024-00680-z

Gribonika, I. et al. Migratory CD103+CD11b+ cDC2s in Peyer’s patches are critical for gut IgA responses following oral immunization. Mucosal Immunology, Volume 17, Issue 4, 509 - 523, https://doi.org/10.1016/j.mucimm.2024.03.004

Rodin, W., Szeponik, L., Rangelova, T. et al. γδ T cells in human colon adenocarcinomas comprise mainly Vδ1, Vδ2, and Vδ3 cells with distinct phenotype and function. Cancer Immunol Immunother 73, 174 (2024). https://doi.org/10.1007/s00262-024-03758-7

[PREPRINT] Bonstingl, L. et al. Circulating tumor cell characterization and classification by novel combinatorial dual-color (CoDuCo) in situ hybridization and supervised machine learning, bioRxiv 2024.05.08.592946; https://doi.org/10.1101/2024.05.08.592946

Andric, F.; Al-Fairouzi, A.; Wettergren, Y.; Szeponik, L.; Bexe-Lindskog, E.; Cusack, J.C., Jr.; Tumusiime, G.; Quiding-Järbrink, M.; Ljungman, D. Immune Microenvironment in Sporadic Early-Onset versus Average-Onset Colorectal Cancer. Cancers 2023, 15, 1457. https://doi.org/10.3390/cancers15051457

[PREPRINT] Olivia Nonn, Olivia Debnath, Daniela S. Valdes et.al., Disturbed trophoblast transition links preeclampsia progression from placenta to the maternal syndrome, bioRxiv preprint doi: https://doi.org/10.1101/2022.10.10.511539

Liang, F.; Nilsson, L.M.; Byvald, F.; Rezapour, A.; Taflin, H.; Nilsson, J.A.; Yrlid, U. A Fraction of CD8+ T Cells from Colorectal Liver Metastases Preferentially Repopulate Autologous Patient-Derived Xenograft Tumors as Tissue-Resident Memory T Cells. Cancers 2022, 14, 2882. https://doi.org/10.3390/cancers14122882

Caër C, Gorreja F, Forsskåhl SK, Brynjolfsson SF, Szeponik L, Magnusson MK, Börjesson LG, Block M, Bexe-Lindskog E, Wick MJ. TREM-1+ Macrophages Define a Pathogenic Cell Subset in the Intestine of Crohn's Disease Patients. J Crohns Colitis. 2021 Aug 2;15(8):1346-1361. https://doi.org/10.1093/ecco-jcc/jjab022

Glise L, Rutberg M, Håversen L, Levin MC, Levin M, Jeppsson A, Borén J, Fogelstrand P. pH-Dependent Protonation of Histidine Residues Is Critical for Electrostatic Binding of Low-Density Lipoproteins to Human Coronary Arteries. Arterioscler Thromb Vasc Biol. 2022 Aug;42(8):1037-1047. doi: 10.1161/ATVBAHA.122.317868. Epub 2022 Jun 2. PMID: 35652335. https://doi.org/10.1161/ATVBAHA.122.317868

Alsén S, Cervin J, Deng Y, Szeponik L, Wenzel UA, Karlsson J, Cucak H, Livingston M, Bryder D, Lu Q, Johansson-Lindbom B and Yrlid U (2022) Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response. Front. Immunol. 13:813203. https://doi.org/ 10.3389/fimmu.2022.813203

Liang, F.; Rezapour, A.; Szeponik, L.; Alsén, S.; Wettergren, Y.; Bexe Lindskog, E.; Quiding-Järbrink, M.; Yrlid, U. Antigen Presenting Cells from Tumor and Colon of Colorectal Cancer Patients Are Distinct in Activation and Functional Status, but Comparably Responsive to Activated T Cells. Cancers 2021, 13, 5247. https://doi.org/10.3390/cancers13205247

Eduard Figueras et. al., Octreotide Conjugates for Tumor Targeting and Imaging, Pharmaceutics 2019, 11, 220; https://doi.org/10.3390/pharmaceutics11050220

Håversen, L., Sundelin, J.P., Mardinoglu, A. et al. Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice. Sci Rep 8, 16973 (2018). https://doi.org/10.1038/s41598-018-34659-2

Yrlid U, Holm M, Levin M, Alsén S, Lindbom M, Glise L, Bergh N, Borén J, Fogelstrand P. Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density. J Leukoc Biol. 2019 Jan;105(1):195-202. doi: 10.1002/JLB.4A1017-402RR. Epub 2018 Sep 28. PMID: 30265749. https://doi.org/10.1002/JLB.4A1017-402RR

Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

S. Kijani, A. M. Vázquez, M. Levin, J. Borén, P. Fogelstrand. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis, Physiol Rep, 5 (14), 2017, e13334, https://doi.org/10.14814/phy2.13334

** Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P (2015) Filter-Dense Multicolor Microscopy. PLoS ONE 10(3): e0119499. https://doi.org/10.1371/journal.pone.0119499

** The original publication demonstrating what is now our SpectraSplit® bandpass filters for multiplex immunofluorescence microscopy.

Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!

 

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