StreptaClick® for Multiplex Immunofluorescence
Multiplex Immunofluorescence Made Easy
Multiplex immunostainings can be time consuming, but the StreptaClick® technology is here to help. StreptaClick® is a monovalent streptavidin that rapidly and firmly binds biotinylated antibodies in solution – without causing antibody aggregation. Simply pre-mix biotinylated antibodies with StreptaClick® Color for rapid assay setup with fluorophores in single tube multiplexing, or StreptaClick® HRP for sequential multiplex staining with 5-10X signal amplification. With either solution you obtain directly conjugated antibodies in minutes without wash steps. Multiple StreptaClick® labeled primary antibodies can then be used for multiplex immunohistochemistry (IHC). The protocols are fast and convenient both when prepared manually or on autostainers.
StreptaClick® Color Workflow
StreptaClick® HRP Workflow
How it works
Biotinylated antibodies pre-mixed with StreptaClick® operate similarly to directly conjugated antibodies, thereby eliminating the need for secondary antibodies. The StreptaClick® labeling reaction is extremely efficient, permitting the labeling of any antibody volume, even down to microliters. This is particularly beneficial when setting up new experiments.
Quick Results, Less Hands On Time
StreptaClick® Color enables a multiplex immunofluorescence (mIF) protocol—from labeling to imaging—to be completed within one hour. Each fluorochrome is attached to its biotinylated antibody with a one-step, 5-minute StreptaClick® reaction. All StreptaClick® labeled antibodies can then be combined and added to the slide simultaneously. StreptaClick® Color is also compatible with flow cytometry.
With StreptaClick® HRP you can complete a 7-color Tyramide Signal Amplification (TSA) multiplex IHC within a single day (DAPI + 6 biotinylated antibodies). Each biotinylated antibody is labeled with HRP by the fast StreptaClick® reaction, and the kit includes an HRP block buffer that efficiently quenches HRP activity between staining cycles. This avoids the slow, inconvenient and harsh boiling step to strip off antibodies required by other solutions between staining cycles. Added benefits of the HRP block buffer include preserved tissue morphology, higher attainable protein biomarker multiplexing levels and a protocol that is automation-friendly.
Seven-Color TSA method for frozen sections from mice, humans, rats, and other species.
The StreptaClick® HRP solution is a must for any manual or automated TSA staining. The kit comes with a HRP quenching buffer that eliminates the need for boiling, which not only accelerates each color cycle but also makes the kit highly beneficial for frozen tissue sections. The quenching buffer effectively silences the HRP signal from the previous round while preserving the morphology of the frozen tissue. Hence, the StreptaClick® HRP kit provides a rapid method for multiplex TSA IHC on both FFPE and frozen sections, with antibodies from any host, and on tissues from mice, humans, rats, and other species.
Automation is also facilitated by the rapid protocol and unique features. The use of primary antibodies and our HRP quenching buffer makes it especially amenable for automated staining. External partners have developed a protocol on the Leica Biosystems BOND RX IHC staining system, and protocols for other systems are in development.
Exceptional Fluorochromes
All our fluorochromes have been carefully selected for their outstanding brightness and signal intensity to enhance image quality and scanning speed. For TSA, we offer an optional selection of eight optimized tyramide dyes that feature remarkable signal intensity, delivering a signal 5-10x brighter than direct conjugates. StreptaClick® Color comes with green, yellow, red, and far red fluorochromes (StreptaClick® Color 488, StreptaClick® Color 542, StreptaClick® Color 594, and StreptaClick® Color 647).
Imaging
The fluorochromes we provide are carefully chosen to align with most common filter sets. However, the inclusion of more fluorochromes in your immunostainings adds complexity. For reliable imaging of tissues stained with up to seven colors (7-plex IHC), we offer a patented combination of filter sets— SpectraSplit® 7, which accommodates all the channels in the chart. SpectraSplit® 7 performs excellently with AZDyes and a variety of commercial dyes, such as AlexaFluor® dyes, FITC, TRITC, Cy3, Cy5, Cy7 dyes, and many of the OpalTM dyes.
| Channel | Traditional dyes | StreptaClick® dyes |
| S-Split Blue (375) | DAPI | DAPI, Tyramide 405 |
| S-Split Cyan (435) | CFP | Tyramide CF®430 |
| S-Split Green (490) | FITC | Tyramide 488, Color 488 |
| S-Split Orange (545) | Cy3/TRITC | Tyramide 555, Color Atto 542 |
| S-Split Red (590) | Texas Red | Tyramide 594, Color 594 |
| S-Split Far-red (650) | Cy5/Cy5.5 | Tyramide 647, Color 647 |
| S-Split Infra-red (740) | Cy7 | Styramide iFluor 750 |
BiotinPure™ Antibody Biotinylation Kit
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SpectraSplit® 10 filter sets (COMING SOON)
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
