The SpectraSplit® 7 filter sets are highly efficient in separating the emission signals of seven different fluorochrome classes, with spillover between channels being less than 0.5%. As a result, high-contrast images are generated without the need for spectral unmixing or any other bleed-through corrections. Post-processing techniques such as bleedthrough corrections can be time consuming, require single-labeled controls, and may increase the risk of over- or undercorrections. In contast, SpectraSplit® 7 directly blocks bleed-through noise and therefore does not require any post processing.