StreptaClick® Precision

400 3694 

StreptaClick® Precision is developed for site-specific conjugation with a defined number of attachment groups for click chemistry – currently exactly one azide for the possibility of achieving a 1:1 ratio of reporter to binder. Furthermore, StreptaClick® Precision has a unique cis-divalent design with maximum separation of the site for conjugation and biotin binding. This design makes StreptaClick® Precision ideal for attachment of bulky molecules without affecting the biotin binding affinity and without causing aggregation when mixed with biotinylated proteins as it still exhibits monovalent binding properties.

StreptaClick® Precision is currently provided with a single azide group for either Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. We observe the highest efficiencies when utilizing SPAAC to react azide to dibenzylcyclooctyne (DBCO), especially when clicking oligonucleotides to StreptaClick® Precision.

Off-the shelf StreptaClick® Precision constructs are also available. These are pre-conjugated with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm).

StreptaClick® Precision with a DBCO group for copper free click chemistry as well as pre-conjugated flurorescent proteins (phycoerythrin), enzymes (ex. horseradish peroxidase (HRP)) are in development. To achieve greater reporter to binder ratios we are also developing versions with exactly three and seven reactive groups.

External partners have successfully attached other molecules, including lipid nanoparticles (LNPs).

Contact us for further information

StreptaClick® Precision - 1 Azide 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 0.1 mg
400 

In stock

StreptaClick® Precision - 1 Azide 0.5 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 0.5 mg
913 

In stock

StreptaClick® Precision - 1 Azide 2 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 2 mg
2463 

In stock

StreptaClick® Precision - Oligo 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 0.1 mg
605 

In stock

StreptaClick® Precision - Oligo 0.5 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 0.5 mg
1370 

In stock

StreptaClick® Precision - Oligo 2 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 2 mg
3694 

In stock

Total items: 0

Total price: 0

Description
Cat no. Product
81Z0101-1 StreptaClick® Precision – 1 Azide 0.1 mg
81Z0501-1 StreptaClick® Precision – 1 Azide 0.5 mg
81Z2X01-1 StreptaClick® Precision – 1 Azide 2 mg
81N0101-1 StreptaClick® Precision – 1 Oligo 0.1 mg
81N0201-1 StreptaClick® Precision – 1 Oligo 0.5 mg
81N2X01-1 StreptaClick® Precision – 1 Oligo 2 mg

StreptaClick® Precision Workflow

Monovalently binding, azide functionalized streptavidin with reporter attachment by precise Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry

How it works

Easily attach the azide labeled StreptaClick® Precision molecule to your reporter molecule of choice via click chemistry. The azide functionalization enables either Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry, ensuring accurate and specific labeling in many different types of experiments. We primarily recommend SPAAC with DBCO- functionalized reporters, but alkyne functionalized reporters also work well.

Attach StreptaClick® Precision conjugate to any biotinylated antibody or surface

After the click chemistry reaction, add the newly formed StreptaClick® Precision construct to your biotinylated protein or surface of interest for near immediate binding. We also have a convenient biotinylation kit available for purchase, BiotinPureTM, if you lack access to biotinylated antibodies.
Additional information
StreptaClick Precision

StreptaClick® Precision – 1 Azide 0.1 mg

,

StreptaClick® Precision – 1 Azide 0.5 mg

,

StreptaClick® Precision – 1 Azide 2 mg

,

StreptaClick® Precision – Oligo 0.1 mg

,

StreptaClick® Precision – Oligo 0.5 mg

,

StreptaClick® Precision – Oligo 2 mg

Publications

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