StreptaClick® Precision

400 3694 

StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution.

StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide).

Pre-conjugated StreptaClick® Precision products are also available. These include StreptaClick® Precision pre-conjugated with a single 14mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm).

Order or request further information

 

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.

We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

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Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.

At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.

NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).

Select your StreptaClick® Precision Kit:

StreptaClick® Precision - 1 DBCO 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - 1 DBCO 0.1 mg
400 

In stock

StreptaClick® Precision - 1 DBCO 0.5 mg
StreptaClick® Precision - StreptaClick® Precision - 1 DBCO 0.5 mg
913 

In stock

StreptaClick® Precision - 1 DBCO 2 mg
StreptaClick® Precision - StreptaClick® Precision - 1 DBCO 2 mg
2463 

In stock

StreptaClick® Precision - 1 Azide 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 0.1 mg
400 

In stock

StreptaClick® Precision - 1 Azide 0.5 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 0.5 mg
913 

In stock

StreptaClick® Precision - 1 Azide 2 mg
StreptaClick® Precision - StreptaClick® Precision - 1 Azide 2 mg
2463 

In stock

StreptaClick® Precision - Oligo 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 0.1 mg
605 

In stock

StreptaClick® Precision - Oligo 0.5 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 0.5 mg
1370 

In stock

StreptaClick® Precision - Oligo 2 mg
StreptaClick® Precision - StreptaClick® Precision - Oligo 2 mg
3694 

In stock

StreptaClick® Precision - 1 488 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - 1 488 0.1 mg
490 

In stock

StreptaClick® Precision - 1 647 0.1 mg
StreptaClick® Precision - StreptaClick® Precision - 1 647 0.1 mg
490 

In stock

Total items: 0

Total price: 0

Description

Ordering information

Cat no. Product
81Z0101-1 StreptaClick® Precision – 1 Azide 0.1 mg
81Z0501-1 StreptaClick® Precision – 1 Azide 0.5 mg
81Z2X01-1 StreptaClick® Precision – 1 Azide 2 mg
81D0101-1 StreptaClick® Precision – 1 DBCO 0.1 mg
81D0501-1 StreptaClick® Precision – 1 DBCO 0.5 mg
81D2X01-1 StreptaClick® Precision – 1 DBCO 2 mg
81N0101-1 StreptaClick® Precision – 1 Oligo 0.1 mg
81N0201-1 StreptaClick® Precision – 1 Oligo 0.5 mg
81N2X01-1 StreptaClick® Precision – 1 Oligo 2 mg
81F4880101-1 StreptaClick® Precision – 1 488 0.1 mg
81F6470101-1 StreptaClick® Precision – 1 647 0.1 mg

How it works

Easily conjugate the reporter molecule of your choice to StreptaClick® Precision via click chemistry. StreptaClick® Precision with 1 DBCO is used with copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). StreptaClick® Precision with 1 Azide can be used for both copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and SPAAC. For oligonucleotides, SPAAC click chemistry (azide/DBCO) is strongly recommended.

StreptaClick® Precision Workflow

Achieve high conjugation efficiency

Conjugating reporters to StreptaClick® Precision via CuAAC or SPAAC click chemistry typically results in 90-100% labeling efficiency with only 1.3X excess of reporter to StreptaClick® molecules.
StreptaClick Precision oligo PAGE gel 250317
Gel photo comparing StreptaClick® – oligo conjugations to controls
  1. StreptaClick® Precision – Oligo
  2. Control: StreptaClick® Precision – DBCO
  3. StreptaClick® Precision – DBCO + 43 bp oligo – azide
  4. StreptaClick® Precision – DBCO + 41 bp oligo – azide
  5. StreptaClick® Precision – DBCO + 37 bp oligo – azide
  6. Control: StreptaClick® Precision – Azide
  7. StreptaClick® Precision – Azide + 20 bp oligo – DBCO
  8. StreptaClick® Precision – Azide + 20 bp oligo – DBCO

Attach StreptaClick® Precision to any biotinylated antibody or surface

After the click chemistry reaction, the StreptaClick® construct can be attached to biotinylated proteins, such as biotinylated antibodies, or biotinylated surfaces. A convenient biotinylation kit is available for antibody biotinylation, BiotinPure™.

Example of immunofluorescence result for StreptaClick® Precision - 1 Oligo

The image below shows B cells in a frozen tissue section of mouse spleen. StreptaClick® Precision - 1 Oligo was first mixed with a 647 fluorochrome antisense oligo and then mixed with biotinylated anti-B220 antibody (Biolegend, clone RA3-6B2). The StreptaClick®-antibody complex was applied to the tissue section for 30 minutes and imaged under a microscope. Other possible readouts for antibody-oligonucleotide based methods include q-PCR and DNA sequencing.
StreptaClick® Precision - 1 Oligo bound to an anti B220 biotinylated antibody

In development

  • StreptaClick® Precision – 1 Azide with fluorochrome tracer
  • Multiarm Azide (3 arms)
  • Phycoerythrin (PE) and horseradish peroxidase (HRP)

Request further information

Additional information
StreptaClick Precision

StreptaClick® Precision – 1 DBCO 0.1 mg

,

StreptaClick® Precision – 1 DBCO 0.5 mg

,

StreptaClick® Precision – 1 DBCO 2 mg

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StreptaClick® Precision – 1 Azide 0.1 mg

,

StreptaClick® Precision – 1 Azide 0.5 mg

,

StreptaClick® Precision – 1 Azide 2 mg

,

StreptaClick® Precision – Oligo 0.1 mg

,

StreptaClick® Precision – Oligo 0.5 mg

,

StreptaClick® Precision – Oligo 2 mg

,

StreptaClick® Precision – 1 488 0.1 mg

,

StreptaClick® Precision – 1 647 0.1 mg

Publications

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