About Us
At Kromnigon, we build imaging workflows with optical separation by design. Together with streptavidin-based precision reagents and heat-free multiplex TSA staining workflows, this enables multicolor imaging with well-preserved tissue morphology. The result is unprocessed images with distinct channel separation - what you see is what you collect.
Who we are
Kromnigon is a biotech company developing smarter tools for multicolor fluorescence microscopy and multiplex immunohistochemistry.
Founded in 2015 by Per Fogelstrand and Ulf Yrlid, Kromnigon is based at the AstraZeneca BioVentureHub in Gothenburg, Sweden. The company grew out of hands-on research experience in histology, immunology, and tissue imaging – where the limitations of existing multiplex workflows were encountered firsthand.
Our principle: what you see is what you collect
In multiplex tissue imaging, confidence depends on clean fluorescent signals.
We design for fluorescence signals to be separated in the optics—not corrected after acquisition by computational redistribution of signal. When signal is reassigned after imaging, interpretation becomes more model-dependent and harder to trust.
This approach guides everything we build: what you see is what you collect – and what you build your data on.
Designed by researchers, for researchers
We make products around real workflow needs that we experienced working in research laboratories:
- Clean optical channel separation without spectral unmixing as a core dependency
- Bright multiplex staining without repeated boiling that otherwise deteriorates tissue morphology
- Faster cycles and no need for antibody order optimization when building new panels
- Increased multiplexing, up to 8–9 plex TSA in FFPE, — and extends multiplex TSA to frozen tissue workflows
- Flexibility to work with a broad range of fluorochromes, without vendor lock-in
What we build
Kromnigon develops an integrated ecosystem for multiplex tissue imaging.
Heat-free multiplex TSA (StreptaClick® HRP)
StreptaClick® HRP enables multiplex tyramide signal amplification without repeated heat treatment. By combining monovalent streptavidin engineering with enzymatic HRP quenching, the workflow preserves tissue integrity and maintains staining consistency across cycles.
Optical separation without spectral unmixing (SpectraSplit®)
SpectraSplit® filter sets separate fluorochromes at the optical level using carefully engineered fixed bandpass filters. This enables clean, interpretable imaging without relying on spectral unmixing or algorithmic correction after acquisition.
Precise biotin binding (StreptaClick® Precision)
StreptaClick® Precision is designed for workflows where controlled, stoichiometric biotin binding matters—supporting applications that demand molecular-level engineering accuracy.
Our direction
Our long-term focus is to help laboratories move multiplex imaging from expert-only workflows to routine deployment—without compromising tissue integrity, data quality, or interpretability.
Core team
Per Fogelstrand, PhD
CEO & Co-founder
Per co-founded Kromnigon in 2015 and leads the company’s product strategy, partnerships, and platform development. He previously headed a research group at the University of Gothenburg and has extensive hands-on experience in histology and multiplex tissue workflows. In parallel with his CEO role, he drives the development of SpectraSplit® 10 for clean fluorochrome separation without spectral unmixing..
Ulf Yrlid, Professor
Scientific Advisor, Co-founder & Business Development
Ulf co-founded Kromnigon in 2015 and supports the company as Scientific Advisor, contributing expertise in immunology, translational research, and assay design. He is Professor in Mucosal Immunology and Microbiology at the University of Gothenburg and has authored more than sixty scientific publications. He is also actively involved in marketing and business development, helping position Kromnigon’s technologies for broader adoption and strategic collaborations.
Anandi Rajan, PhD
R&D Manager
Anandi leads R&D and product development at Kromnigon, focusing on translating advanced technologies into robust and reproducible protocols. She holds a PhD from Umeå University and conducted postdoctoral research at the University of Gothenburg, with a strong background in molecular biology and infection biology. Her extensive experience in protein engineering, conjugation and characterization ensures that StreptaClick® Precision continues to expand through clever, user-driven product design.
Johan Vänelid, PhLic
Director of Sales
Johan leads Kromnigon’s commercial strategy and customer engagement, helping laboratories and CROs adopt multiplex workflows that scale. Since 2012 he has worked commercially in roles focused on establishing new business area in companies ranging from startups to Roche Diagnostics. Johan holds a PhLic from Uppsala University and the Royal Institute of Technology (KTH) in molecular- and immunoassay tools development, including multiplex proximity ligation assays. This combination of academic depth and commercial experience enables him to understand customer workflow-level needs and translate them into the right technical solution.
Location
Kromnigon is located within the premises of AstraZeneca R&D (Gothenburg, Sweden)
Our Partners
Gobia enterprises is a family-owned investment company with decades of experience in building companies and developing ideas from research into product offerings and worldwide sales. Gobia enterprises has extensive knowledge about life science industry, and an extensive international network.
Västkustens Affärsänglar was founded in 2011 by entrepreneurs and business leaders to invest in start-ups ready to take the next step in development and growth.
Almi Invest is owned by the Swedish government. It is a venture capital company that invests in start ups throughout Sweden via eight regional venture capital companies.
Västra Götalandsregionen (VGR) works to create a vivid and sustainable industry. The goal is job creation by entrepreneurship and corporate growth. VGR works toward this goal by helping companies with seed capital and free consulting.
