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Documentation, videos and publications

DOCUMENTATION (SpectraSplit®)

SpectraSplit® 7

Folder
SpectraSplit® 7 folder

DOCUMENTATION (StreptaClick®)

StreptaClick® HRP Multiplex IHC kit

Flyer
StreptaClick® HRP flyer

User Manual
User Manual StreptaClick® HRP

SDS

SDS EN SCHRP KIT – Antibody labeling reagent (StreptaClick HRP)_2.1_60810

SDS EN SCHRP KIT – 3% H2O2_2.1_60806

SDS EN SCHRP KIT – Biotin block buffer_2.1_60804

SDS EN SCHRP KIT – Block activator_v2

SDS EN SCHRP KIT – HRP block buffer_2.1_60805

Tyramide dyes

User Manual
Manual Tyramide Dye kit

SDS

SDS EN Tyramide dyes_1.0_76444

Tyramide Signal Amplification

User Manual
Manual Tyramide Dye kit

SDS
SDS Biotium Tyramide Amplification Buffer PLUS

StreptaClick® Color

Flyer
StreptaClick® Color flyer

User Manual
User Manual StreptaClick® Color

SDS
SDS EN Antibody labeling reagent (StreptaClick® Color)

SDS EN Biotin block buffer

StreptaClick® Precision

Flyer
StreptaClick® Precision flyer

User Manual
Manual StreptaClick® Precision – CuAAC

Manual StreptaClick® Precision – SPAAC

BiotinPure™ Antibody Biotinylation Kit

Flyer
BiotinPure™ flyer

User Manual
Manual BiotinPure™ Antibody Biotinylation kit

SDS

SDS EN BIOTINPURE KIT – 10x Reaction buffer_1.0

SDS EN BIOTINPURE KIT – 10x Wash buffer_1.0

SDS EN BIOTINPURE KIT – Biotinylation reagent_1.0_76433

SDS EN BIOTINPURE KIT – DMSO_1.0_76432

DOCUMENTATION (OTHER)

Protocol for preparation of frozen tissue sections

Protocol
Preparation frozen tissues

VIDEOS

StreptaClick® Color

Video StreptaClick® Color

StreptaClick® HRP Complete TSA IHC Kit

Video StreptaClick® HRP

PUBLICATIONS

SpectraSplit® 7

2026

Emiel Slaats et.al., Single Nucleotide Polymorphism Typing Going Spatial: In Situ Padlock Probes Targeting mRNA Variants to Identify Haploidentical Cells within the Tissue Environment, Clinical Chemistry, Volume 72, Issue 1, January 2026, Pages 163–172, http://doi.org/10.1093/clinchem/hvaf119

2025

[PREPRINT] Stefan Kühberger et.al., Tissue architecture and immune niches govern ctDNA release in colorectal cancer, bioRxiv preprint doi: https://doi.org/10.1101/2025.11.28.691139

Alexander Potthoff et. Al., Spatial biology using single-cell mass spectrometry imaging and integrated microscopy, Nature Communications volume 16, Article number: 9129 (2025), https://doi.org/10.1038/s41467-025-64603-8

[PREPRINT] Sebastian Bessler et.al., Single-cell mass spectrometry imaging combined with immunofluorescence reveals neutrophil heterogeneity in inflammation, bioRxiv preprint doi: https://doi.org/10.1101/2025.08.19.671021

Min Peng et.al., RAD51 is chromatin enriched and targetable in BRCA1-deficient cells, Molecular Cell, Volume 85, Issue 18, 3373 – 3387.e6, https://doi.org/10.1016/j.molcel.2025.08.017

Adam J. Fike AJ, et. al. IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints, J Exp Med (2025) 222 (7): e20231882, https://doi.org/10.1084/jem.20231882

Luis Enrique Sastré-Velásquez et.al., Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers, Journal of Biological Engineering (2025) 19:69, https://doi.org/10.1186/s13036-025-00539-3

[PREPRINT] Sukanya Iyer, Katelyn Daman, Yehui Sun et. al., Efficient Cas9 nuclease-based editing in skeletal muscle via lipid nanoparticle delivery., bioRxiv preprint doi: https://doi.org/10.1101/2025.05.12.653518

Elena Garre, Sara Rhost, Anna Gustafsson, Louis Szeponik, Thais Fenz Araujo, Marianne Quiding-Järbrink, Khalil Helou, Anders Ståhlberg & Göran Landberg, Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity, Communications Biology | ( 2025) 8:621, https://doi.org/10.1038/s42003-025-08054-3

[PREPRINT] Fike AJ, Bricker KN, Gonzalez MV, et. al. IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints. bioRxiv [Preprint]. 2025 Feb 8:2025.02.04.636277. doi: 10.1101/2025.02.04.636277

2024

Bonstingl, L., Zinnegger, M., Sallinger, K. et al. Advanced single-cell and spatial analysis with high-multiplex characterization of circulating tumor cells and tumor tissue in prostate cancer: Unveiling resistance mechanisms with the CoDuCo in situ assay. Biomark Res 12, 140 (2024). https://doi.org/10.1186/s40364-024-00680-z

Gribonika, I. et al. Migratory CD103+CD11b+ cDC2s in Peyer’s patches are critical for gut IgA responses following oral immunization. Mucosal Immunology, Volume 17, Issue 4, 509 – 523, https://doi.org/10.1016/j.mucimm.2024.03.004

Rodin, W., Szeponik, L., Rangelova, T. et al. γδ T cells in human colon adenocarcinomas comprise mainly Vδ1, Vδ2, and Vδ3 cells with distinct phenotype and function. Cancer Immunol Immunother 73, 174 (2024). https://doi.org/10.1007/s00262-024-03758-7

[PREPRINT] Bonstingl, L. et al. Circulating tumor cell characterization and classification by novel combinatorial dual-color (CoDuCo) in situ hybridization and supervised machine learning, bioRxiv 2024.05.08.592946; https://doi.org/10.1101/2024.05.08.592946

2023

Andric, F.; Al-Fairouzi, A.; Wettergren, Y.; Szeponik, L.; Bexe-Lindskog, E.; Cusack, J.C., Jr.; Tumusiime, G.; Quiding-Järbrink, M.; Ljungman, D. Immune Microenvironment in Sporadic Early-Onset versus Average-Onset Colorectal Cancer. Cancers 2023, 15, 1457. https://doi.org/10.3390/cancers15051457

2022

[PREPRINT] Olivia Nonn, Olivia Debnath, Daniela S. Valdes et.al., Disturbed trophoblast transition links preeclampsia progression from placenta to the maternal syndrome, bioRxiv preprint doi: https://doi.org/10.1101/2022.10.10.511539

Liang, F.; Nilsson, L.M.; Byvald, F.; Rezapour, A.; Taflin, H.; Nilsson, J.A.; Yrlid, U. A Fraction of CD8+ T Cells from Colorectal Liver Metastases Preferentially Repopulate Autologous Patient-Derived Xenograft Tumors as Tissue-Resident Memory T Cells. Cancers 2022, 14, 2882. https://doi.org/10.3390/cancers14122882

Caër C, Gorreja F, Forsskåhl SK, Brynjolfsson SF, Szeponik L, Magnusson MK, Börjesson LG, Block M, Bexe-Lindskog E, Wick MJ. TREM-1+ Macrophages Define a Pathogenic Cell Subset in the Intestine of Crohn’s Disease Patients. J Crohns Colitis. 2021 Aug 2;15(8):1346-1361. https://doi.org/10.1093/ecco-jcc/jjab022

Glise L, Rutberg M, Håversen L, Levin MC, Levin M, Jeppsson A, Borén J, Fogelstrand P. pH-Dependent Protonation of Histidine Residues Is Critical for Electrostatic Binding of Low-Density Lipoproteins to Human Coronary Arteries. Arterioscler Thromb Vasc Biol. 2022 Aug;42(8):1037-1047. doi: 10.1161/ATVBAHA.122.317868. Epub 2022 Jun 2. PMID: 35652335. https://doi.org/10.1161/ATVBAHA.122.317868

Alsén S, Cervin J, Deng Y, Szeponik L, Wenzel UA, Karlsson J, Cucak H, Livingston M, Bryder D, Lu Q, Johansson-Lindbom B and Yrlid U (2022) Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response. Front. Immunol. 13:813203. https://doi.org/ 10.3389/fimmu.2022.813203

2021

Liang, F.; Rezapour, A.; Szeponik, L.; Alsén, S.; Wettergren, Y.; Bexe Lindskog, E.; Quiding-Järbrink, M.; Yrlid, U. Antigen Presenting Cells from Tumor and Colon of Colorectal Cancer Patients Are Distinct in Activation and Functional Status, but Comparably Responsive to Activated T Cells. Cancers 2021, 13, 5247. https://doi.org/10.3390/cancers13205247

2019

Eduard Figueras et. al., Octreotide Conjugates for Tumor Targeting and Imaging, Pharmaceutics 2019, 11, 220; https://doi.org/10.3390/pharmaceutics11050220

2018

Håversen, L., Sundelin, J.P., Mardinoglu, A. et al. Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice. Sci Rep 8, 16973 (2018). https://doi.org/10.1038/s41598-018-34659-2

Yrlid U, Holm M, Levin M, Alsén S, Lindbom M, Glise L, Bergh N, Borén J, Fogelstrand P. Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density. J Leukoc Biol. 2019 Jan;105(1):195-202. doi: 10.1002/JLB.4A1017-402RR. Epub 2018 Sep 28. PMID: 30265749. https://doi.org/10.1002/JLB.4A1017-402RR

2017

Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

S. Kijani, A. M. Vázquez, M. Levin, J. Borén, P. Fogelstrand. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis, Physiol Rep, 5 (14), 2017, e13334, https://doi.org/10.14814/phy2.13334

** Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P (2015) Filter-Dense Multicolor Microscopy. PLoS ONE 10(3): e0119499. https://doi.org/10.1371/journal.pone.0119499

** The original publication demonstrating what is now our SpectraSplit® bandpass filters for multiplex immunofluorescence microscopy.

StreptaClick®

2025

Andersson E, et al., Evaluation of Antibody Labeling kits for Multiplexed Imaging of Tissue Sections [Internet] [Dissertation]. 2025. (TRITA-CBH-GRU). Available from: https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-371225

2020

Thomas Naessens, Yannick Morias, Eva Hamrud et. Human Lung Conventional Dendritic Cells Orchestrate Lymphoid Neogenesis during Chronic Obstructive Pulmonary Disease, al., American Journal of Respiratory and Critical Care Medicine Volume 202 Number 4 | August 15 2020, https://doi.org/10.1164/rccm.201906-1123OC

Manuela Gast et al. , Use of Super-Resolution Optical Microscopy To Reveal Direct Virus Binding at Hybrid Core–Shell Matrixes. Anal. Chem. 2020, 92, 3050−3057 https://dx.doi.org/10.1021/acs.analchem.9b04328?ref=pdf

2017

**Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

**The first publication in which StreptaClick® was used as a tool, making it to the front page of Science Immunology. At the time it was referred to as FlexiStain, the first version of StreptaClick® Color.

POSTERS

*** Enhancing Spatial Biology with Kromnigon’s StreptaClick® Technology and ZEISS Axioscan 7

*** Poster presented by ZEISS at SITC (Society for Immunotherapy of Cancer) 2024 and ESSB (European Society for Spatial Biology) 2024 displaying the results of a joint project with ZEISS in which a co-optimized ZEISS Axioscan 7 microscope, combined with StreptaClick® technology, enabled high throughput multiplex immunofluorescence imaging with 8-channel separation without the need for spectral unmixing.