Research collaboration between arcoris bio and Kromnigon for parallel, high-sensitivity multiplex immunofluorescence using biotinylated primary antibodies

Parallel, high-sensitivity multiplex immunofluorescence using biotinylated primary antibodies

Biotinylated primary antibodies can now be integrated into a parallel, scalable, cycle-free multiplex immunofluorescence (mIF) workflow.

By combining Kromnigon’s StreptaClick® Precision, a modified streptavidin for monovalent biotin binding and optimized for conjugation to a single reporter molecule, with Arcoris’ MUSE enzyme-free signal amplification, multiple targets can be detected with high sensitivity in parallel.

Biotinylated primary antibodies labeled with StreptaClick® Precision – MUSE activator oligo conjugates are pooled and applied simultaneously, followed by parallel amplification of all targets. This approach simplifies and speeds up the workflow while enabling multiplex detection without iterative staining cycles. Additionally, the same biotinylated primary antibodies can be seamlessly used in other StreptaClick workflows, such as StreptaClick HRP heat-free TSA or with ultra-rapid StreptaClick Color direct labeling.

Shown here is a 6-plex analysis of an FFPE tonsil section, displaying the individual marker channels alongside the merged multiplex image.

This represents a new powerful approach for streamlined, high-sensitivity spatial biomarker analysis and the first (public) example of StreptaClick® Precision in combination with a multiplex oligo-based signal amplification technology.

Meet arcoris bio and Kromnigon in person at #ESSB2026 to learn more about this research collaboration.