SpectraSplit® 10 filter sets (COMING APRIL 2026) - Image 1
Click to enlarge

SpectraSplit® 10 filter sets (COMING APRIL 2026)

SpectraSplit® 10, developed from our patented SpectraSplit® 7 filter sets, will revolutionize fluorescence microscopy as 10 channels can be directly imaged without spectral unmixing.  The channels are distributed over five filter sets and separate the most commonly used fluorochromes in immunofluorescence, in addition to channels anticipated to gain popularity in the near future (see table below). Tissues and other sample types can be immunostained with up to 10 fluorochromes, visualized without bleedthrough. No additional software and no computational spectral unmixing are required.

SpectraSplit® 10 can easily be installed in both standard and scanning fluorescence microscopes.

COMING APRIL 2026

Request a quote or information

 

Description

SpectraSplit® 10 - pushing the boundaries of optical physics

The SpectraSplit® 10 filter sets are highly efficient in separating the emission signals of ten different fluorochrome classes, with spillover between channels being less than 0.5% for the majority of the channels. Consequently, high-contrast images for ten distinct channels are generated without the need for spectral unmixing or any other corrections – a unique accomplishment.

SpectraSplit® 10 immediately and uniquely empowers your microscope with ten independent color channels that each generates crystal clear images. Importantly, you do not need to be a fluorochrome expert or microscope guru to produce 10-color images.

Fluorochromes
SpectraSplit® 10 is fully compatible with commonly used fluorochromes, such as AlexaFluor® dyes, as well as the classic FITC/Cy3/Cy5/Cy7 dyes and many of the OpalTM dyes.

In addition, our own StreptaClick® reagents for multiplex IHC have been optimized to be compatible with SpectraSplit® 7 for easy 7-color assay development to make the most of your microscope or scanner equipped with SpectraSplit® 7. We will increase the number of StreptaClick® reagents for the launch of SpectraSplit® 10 to allow users to fully unleash the multiplexing power of the filter sets.

Fluorochromes compatible with SpectraSplit® 10

*Cy3, Cy5, Cy7 are registered trademarks of GE Healthcare, Aluora, Alexa Fluor and DyLight dyes are trademarks of Thermo Fisher Scientific, CF dyes are trademarks of Biotium, Atto dyes are trademarks of Atto-tec, Opal dyes are trademarks of Akoya Biosciences, Brilliant Violet dyes are trademarks of Sirigen/BD

Filter channels
The channels will be at 365, 405, 435, 490, 545, 590, 650, 740 and 850 nm. However, the exact bandwidths are proprietary information and not publicly disclosed.

Microscopes and light sources
SpectraSplit® 10 is designed to work with both standard and scanning fluorescence microscopes.

Contact us if you want to know more or discuss testing SpectraSplit® 10 ahead of launch as one of our early access customers.

Ordering information

Ordering information

Cat no. Product
TBD SpectraSplit® 10

COMING SOON. Please contact us to inquire about custom filter configurations and to learn more ahead of product launch.

Resources

RESOURCES - FOR SpectraSplit® 7

FOLDER
SpectraSplit® 7 folder

PUBLICATIONS

[PREPRINT] Sebastian Bessler et.al., Single-cell mass spectrometry imaging combined with immunofluorescence reveals neutrophil heterogeneity in inflammation, bioRxiv preprint doi: https://doi.org/10.1101/2025.08.19.671021

Luis Enrique Sastré-Velásquez et.al., Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers, Journal of Biological Engineering (2025) 19:69, https://doi.org/10.1186/s13036-025-00539-3

[PREPRINT] Alexander Potthoff et. Al., Spatial biology using single-cell mass spectrometryimaging and integrated microscopy, Research Square Preprint, https://doi.org/10.21203/rs.3.rs-6751055/v1

[PREPRINT] Sukanya Iyer, Katelyn Daman, Yehui Sun et. al., Efficient Cas9 nuclease-based editing in skeletal muscle via lipid nanoparticle delivery., bioRxiv preprint doi: https://doi.org/10.1101/2025.05.12.653518

Elena Garre, Sara Rhost, Anna Gustafsson, Louis Szeponik, Thais Fenz Araujo, Marianne Quiding-Järbrink, Khalil Helou, Anders Ståhlberg & Göran Landberg, Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity, Communications Biology | ( 2025) 8:621, https://doi.org/10.1038/s42003-025-08054-3

[PREPRINT] Fike AJ, Bricker KN, Gonzalez MV, et. al. IRF7 controls spontaneous autoimmune germinal center and plasma cell checkpoints. bioRxiv [Preprint]. 2025 Feb 8:2025.02.04.636277. doi: 10.1101/2025.02.04.636277

Bonstingl, L., Zinnegger, M., Sallinger, K. et al. Advanced single-cell and spatial analysis with high-multiplex characterization of circulating tumor cells and tumor tissue in prostate cancer: Unveiling resistance mechanisms with the CoDuCo in situ assay. Biomark Res 12, 140 (2024). https://doi.org/10.1186/s40364-024-00680-z

Gribonika, I. et al. Migratory CD103+CD11b+ cDC2s in Peyer’s patches are critical for gut IgA responses following oral immunization. Mucosal Immunology, Volume 17, Issue 4, 509 - 523, https://doi.org/10.1016/j.mucimm.2024.03.004

Rodin, W., Szeponik, L., Rangelova, T. et al. γδ T cells in human colon adenocarcinomas comprise mainly Vδ1, Vδ2, and Vδ3 cells with distinct phenotype and function. Cancer Immunol Immunother 73, 174 (2024). https://doi.org/10.1007/s00262-024-03758-7

[PREPRINT] Bonstingl, L. et al. Circulating tumor cell characterization and classification by novel combinatorial dual-color (CoDuCo) in situ hybridization and supervised machine learning, bioRxiv 2024.05.08.592946; https://doi.org/10.1101/2024.05.08.592946

Andric, F.; Al-Fairouzi, A.; Wettergren, Y.; Szeponik, L.; Bexe-Lindskog, E.; Cusack, J.C., Jr.; Tumusiime, G.; Quiding-Järbrink, M.; Ljungman, D. Immune Microenvironment in Sporadic Early-Onset versus Average-Onset Colorectal Cancer. Cancers 2023, 15, 1457. https://doi.org/10.3390/cancers15051457

[PREPRINT] Olivia Nonn, Olivia Debnath, Daniela S. Valdes et.al., Disturbed trophoblast transition links preeclampsia progression from placenta to the maternal syndrome, bioRxiv preprint doi: https://doi.org/10.1101/2022.10.10.511539

Liang, F.; Nilsson, L.M.; Byvald, F.; Rezapour, A.; Taflin, H.; Nilsson, J.A.; Yrlid, U. A Fraction of CD8+ T Cells from Colorectal Liver Metastases Preferentially Repopulate Autologous Patient-Derived Xenograft Tumors as Tissue-Resident Memory T Cells. Cancers 2022, 14, 2882. https://doi.org/10.3390/cancers14122882

Caër C, Gorreja F, Forsskåhl SK, Brynjolfsson SF, Szeponik L, Magnusson MK, Börjesson LG, Block M, Bexe-Lindskog E, Wick MJ. TREM-1+ Macrophages Define a Pathogenic Cell Subset in the Intestine of Crohn's Disease Patients. J Crohns Colitis. 2021 Aug 2;15(8):1346-1361. https://doi.org/10.1093/ecco-jcc/jjab022

Glise L, Rutberg M, Håversen L, Levin MC, Levin M, Jeppsson A, Borén J, Fogelstrand P. pH-Dependent Protonation of Histidine Residues Is Critical for Electrostatic Binding of Low-Density Lipoproteins to Human Coronary Arteries. Arterioscler Thromb Vasc Biol. 2022 Aug;42(8):1037-1047. doi: 10.1161/ATVBAHA.122.317868. Epub 2022 Jun 2. PMID: 35652335. https://doi.org/10.1161/ATVBAHA.122.317868

Alsén S, Cervin J, Deng Y, Szeponik L, Wenzel UA, Karlsson J, Cucak H, Livingston M, Bryder D, Lu Q, Johansson-Lindbom B and Yrlid U (2022) Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response. Front. Immunol. 13:813203. https://doi.org/ 10.3389/fimmu.2022.813203

Liang, F.; Rezapour, A.; Szeponik, L.; Alsén, S.; Wettergren, Y.; Bexe Lindskog, E.; Quiding-Järbrink, M.; Yrlid, U. Antigen Presenting Cells from Tumor and Colon of Colorectal Cancer Patients Are Distinct in Activation and Functional Status, but Comparably Responsive to Activated T Cells. Cancers 2021, 13, 5247. https://doi.org/10.3390/cancers13205247

Eduard Figueras et. al., Octreotide Conjugates for Tumor Targeting and Imaging, Pharmaceutics 2019, 11, 220; https://doi.org/10.3390/pharmaceutics11050220

Håversen, L., Sundelin, J.P., Mardinoglu, A. et al. Vimentin deficiency in macrophages induces increased oxidative stress and vascular inflammation but attenuates atherosclerosis in mice. Sci Rep 8, 16973 (2018). https://doi.org/10.1038/s41598-018-34659-2

Yrlid U, Holm M, Levin M, Alsén S, Lindbom M, Glise L, Bergh N, Borén J, Fogelstrand P. Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density. J Leukoc Biol. 2019 Jan;105(1):195-202. doi: 10.1002/JLB.4A1017-402RR. Epub 2018 Sep 28. PMID: 30265749. https://doi.org/10.1002/JLB.4A1017-402RR

Jayendra Kumar Krishnaswamy et al. , Migratory CD11b+ conventional dendritic cells induce T follicular helper cell–dependent antibody responses.Sci. Immunol.2,eaam9169(2017).DOI:10.1126/sciimmunol.aam9169

S. Kijani, A. M. Vázquez, M. Levin, J. Borén, P. Fogelstrand. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis, Physiol Rep, 5 (14), 2017, e13334, https://doi.org/10.14814/phy2.13334

** Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P (2015) Filter-Dense Multicolor Microscopy. PLoS ONE 10(3): e0119499. https://doi.org/10.1371/journal.pone.0119499

** The original publication demonstrating what is now our SpectraSplit® bandpass filters for multiplex immunofluorescence microscopy.

Is your publication utilizing a Kromnigon product not listed on our site? Please let us know!

 

Related products

BiotinPure™ Antibody Biotinylation Kit

503 €
The BiotinPure™ Antibody Biotinylation Kit is a complete kit for easy biotinylation of antibodies at room temperature within 1 hour. The kit uses amine/ester chemistry to attach 2-3 biotins per antibody and includes a purification wash step that removes unreacted free biotin from the final biotinylated antibody solution. The antibodies to be biotinylated should be free from other proteins, such as BSA. The BiotinPure™ Antibody Biotinylation Kit is designed for use with StreptaClick® reagents. The biotinylated antibodies may also be used for other applications. Cat. no. 9000051-1 Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally - a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your BiotinPure™ Kit:
Select options This product has multiple variants. The options may be chosen on the product page

SpectraSplit® 7

SpectraSplit® 7 are patented filter sets that empower fluorescence microscopes with seven color channels.  The channels are distributed over four filter sets and specifically separate the most commonly used fluorochromes in immunofluorescence (see table below). Hence, you can immunostain tissues with up to seven fluorochromes and still visualize each fluorochrome in the sample with SpectraSplit® 7 without bleedthrough. No additional software and no computational spectral unmixing are required. SpectraSplit® 7 can easily be installed in both standard and scanning fluorescence microscopes, and can be run with different popular light sources. See the description below for more information. Cat no. 7001991-1 Request a quote or information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. We can also charge in $USD. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Read more

StreptaClick® 8-plex for ZEISS Axioscan 7 spatial biology

The StreptaClick® 8-Plex Multiplex IHC Kit provides a rapid and heat-treatment-free Tyramide Signal Amplification (TSA) staining cyclic workflow for frozen and FFPE tissue sections. It is optimized for imaging on the ZEISS Axioscan 7 spatial biology platform, enabling visualization of up to eight colors in a single run. The kit is ideal for researchers in high-throughput laboratories who require a reliable, time-efficient, and flexible multiplex immunohistochemistry workflow.
Contact your local ZEISS representative to learn more
Read more

StreptaClick® Color

Price range: 339 € through 785 €
StreptaClick® Color is a modified streptavidin that labels biotinylated antibodies with fluorochromes in solution, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer. Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
Select options This product has multiple variants. The options may be chosen on the product page

StreptaClick® HRP

Price range: 400 € through 1734 €
StreptaClick® HRP Multiplex IHC Kit is an accelerated heat-treatment-free multiplex Tyramide Signal Amplification (TSA) staining solution with imaging for up to six colors for both frozen and FFPE samples in one day. This comprehensive kit is designed for researchers who demand ease of use, flexibility, precision and reliability in immunohistochemistry (IHC). The kit includes everything needed to label biotinylated antibodies with horseradish peroxidase (HRP) enzyme in solution utilizing our modified streptavidin StreptaClick®. As StreptaClick® removes the issue of antibody aggregation through monovalent biotin binding it is particularly suited for labeling primary biotinylated antibodies. The kit also features a novel HRP block buffer that eliminates the need for antibody-stripping by heat-treatment such as boiling between cycles. This feature maintains tissue morphology over multiple staining cycles making it ideal for both frozen and FFPE tissue sections, and higher degrees of multiplexing than comparable solutions. In combination, these features makes the protocol automation friendly and results for six color + DAPI experiments are achievable in one day.
Order or request further information

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® HRP Kit:
Select options This product has multiple variants. The options may be chosen on the product page

StreptaClick® Precision

Price range: 400 € through 3694 €
StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution. Click-chemistry ready StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide). Pre-conjugated, off-the-shelf StreptaClick® Precision based, pre-conjugated products are also available. These include StreptaClick® Precision with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm). Custom conjugation as a service Please inquire to discuss the feasability of your project. Order or request further information  

Request a quote or order

Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.

Webshop

Orders can also be placed through our webshop via the form below.  Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Precision Kit:
Select options This product has multiple variants. The options may be chosen on the product page