StreptaClick® Color

Price range: 339 € through 785 €

StreptaClick® Color is a modified streptavidin that labels biotinylated antibodies with fluorochromes in solution, mimicking directly conjugated antibodies as we have removed the issue of antibody aggregation through monovalent binding. StreptaClick® Color provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. Once labeled with StreptaClick®, the antibody is stable and can be used in the same manner as antibodies that are directly conjugated with fluorochromes. The labeling reaction is highly efficient and can be carried out at any volume, concentration, or buffer.

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Select your StreptaClick® Color Kit:

StreptaClick® Color 488
StreptaClick® Color - StreptaClick® Color 488
339 

Out of stock

StreptaClick® Color 647
StreptaClick® Color - StreptaClick® Color 647
339 

Out of stock

StreptaClick® Color 2-plex Kit
StreptaClick® Color - StreptaClick® Color 2-plex Kit
585 

Out of stock

StreptaClick® Color Complete 2-plex Kit
StreptaClick® Color - StreptaClick® Color Complete 2-plex Kit
785 

Out of stock

Total items: 0

Total price: 0

Description

StreptaClick®Color Workflow

Unlike any other biotin-streptavidin IHC methods

Attach

Mix each biotinylated antibody with a StreptaClick® Color

Mix

Mix all the different StreptaClick®-antibody conjugates in one tube

Analyze

Add all colors simultanously to the slide for IHC or perform Flow Cytometry

How it works

Biotinylated antibodies pre-mixed with StreptaClick® operate similarly to directly conjugated antibodies, thereby eliminating the need for secondary antibodies. The StreptaClick® labeling reaction is extremely efficient, permitting the labeling of any antibody volume, even down to microliters. This is particularly beneficial when setting up new experiments.

Fast – flexible – multiplex

StreptaClick® Color enables a multiplex immunofluorescence (mIF) protocol—from labeling to imaging—to be completed within one hour. Each fluorochrome is attached to its biotinylated antibody with a one-step, 5-minute StreptaClick® reaction. All StreptaClick® labeled antibodies can then be combined and added to the slide simultaneously. StreptaClick®-antibody conjugates can be combined with DAPI, other directly conjugated primary antibodies or conventional secondary antibodies in the same assay. However, StreptaClick® is not compatible with other streptavidin-based techniques. StreptaClick® Color is also compatible with flow cytometry.

Fluorochrome specifications

Name Absorption max Emission max
StreptaClick® Color 488 – Green 494 nm 517 nm
StreptaClick® Color 647 – Far red 649 nm 671 nm
Additional information
StreptaClick ColorStreptaClick® Color 2-plex Kit, StreptaClick® Color 488, StreptaClick® Color 647, StreptaClick® Color Complete 2-plex Kit
Ordering information

Ordering information

Cat no. Product
5004881-1 StreptaClick® Color 488
5006471-1 StreptaClick® Color 647
50021-1 StreptaClick® Color 2-plex Kit
50022-1 StreptaClick® Color Complete 2-plex Kit

Please contact us to inquire about larger pack sizes and custom kit configurations.

Each StreptaClick® Color contains 500 μl of StreptaClick® antibody labeling reagent, capable of labeling 50 μg of biotinylated antibody, sufficient for approximately 50 slides. The kit also includes a biotin block buffer. This buffer is used to block any remaining free StreptaClick® when multiplexing.

The StreptaClick® Color 2-plex Kit contains the Labeling Reagents for StreptaClick® Color 488 and StreptaClick® Color 647, along with Biotin Block Buffer. The StreptaClick® 2-Color Kit is suitable for multiplex immunohistochemistry (IHC) and Flow Cytometry.

The StreptaClick® Color Complete kits also include our convenient BiotinPure™ Antibody Biotinylation kit in addition to the StreptaClick® Color reagents.