StreptaClick® Precision
Price range: 400 € through 3694 €
StreptaClick® Precision is a modified streptavidin designed for precise stoichiometric 1:1 conjugations. Currently, StreptaClick® Precision is available with exactly one azide or exactly one DBCO (dibenzylcyclooctyne). StreptaClick® Precision also features a unique cis-divalent design for enhanced monovalent binding to biotinylated proteins. In addition, the conjugation site and the biotin binding region are on opposite sides of StreptaClick® Precision, minimizing the risk of conjugates interfering with biotin binding. This design makes StreptaClick® Precision ideal for attaching bulky molecules without sterically hindering biotin binding and without causing aggregation when mixed with biotinylated antibodies in solution.
Click-chemistry ready
StreptaClick® Precision is currently available with a single azide or DBCO group for either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
For oligonucleotides, we strongly recommend SPAAC click chemistry (DBCO/azide).
Pre-conjugated, off-the-shelf
StreptaClick® Precision based, pre-conjugated products are also available. These include StreptaClick® Precision with a single 14 mer DNA oligonucleotide or a single bright fluorochrome (488 nm or 647 nm).
Custom conjugation as a service
Please inquire to discuss the feasability of your project.
Order or request further information
Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier.
We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.
Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout.
At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically.
NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries).
Select your StreptaClick® Precision Kit:
StreptaClick® Precision Workflow
How it works
Achieve high conjugation efficiency
Gel photo comparing StreptaClick® – oligo conjugations to controls
- StreptaClick® Precision – Oligo
- Control: StreptaClick® Precision – DBCO
- StreptaClick® Precision – DBCO + 43 bp oligo – azide
- StreptaClick® Precision – DBCO + 41 bp oligo – azide
- StreptaClick® Precision – DBCO + 37 bp oligo – azide
- Control: StreptaClick® Precision – Azide
- StreptaClick® Precision – Azide + 20 bp oligo – DBCO
- StreptaClick® Precision – Azide + 20 bp oligo – DBCO
Attach StreptaClick® Precision to any biotinylated antibody or surface
After the click chemistry reaction, the StreptaClick® construct can be attached to biotinylated proteins, such as biotinylated antibodies, or biotinylated surfaces. A convenient biotinylation kit is available for antibody biotinylation, BiotinPure™.
Applications
Possible applications include:
- DNA origami, precise labeling of reaction surfaces or beads
- Super-resolution microscopy
- Antibody-oligonucleotide conjugate based immunoassays such as immuno-PCR, immuno-RCA, and Proximity Ligation Assays
- Prototyping or screening antibody-drug conjugates, label free antibody-antigen interaction kinetics for antibody therapeutics
- Screening targeting antibodies conjugated to delivery systems such as lipid nanoparticles or extracelluar vesicles in the development of advanced therapies and medicinal products (ATMPs).
Example of immunofluorescence result for StreptaClick® Precision - 1 Oligo
In development
- StreptaClick® Precision – 1 Azide with fluorochrome “tracer” (three fluorochromes on the streptavidin body) – now available for purchase upon request
- Multiarm Azide (3 or more arms with reactive groups)
- Phycoerythrin (PE) and horseradish peroxidase (HRP)
StreptaClick Precision has greatly facilitated our research based on lipid nanoparticles (LNP).
We study subsets of macrophages using antibody coated LNPs containing functional RNA to
genetically reprogram the subsets through antibody-targeted delivery. We can now simply
generate a large batch of StreptaClick Precision LNPs via click chemistry and then easily mix
this with different biotinylated antibodies from our library to screen for the most suitable
antibodies to use for the targeting.
We have recently evaluated a new version of StreptaClick Precision that features, in
addition to azide for click chemistry, a tracer fluorochrome. This allows us to confirm LNP
uptake by the correct subset as well as correlate this to the expression of target RNA by GFP
or mCherry in another fluorescent channel.
StreptaClick Precision has become a game changer for us as it is convenient, consistent
and flexible compared to directly conjugating each antibody to the LNPs, and also cost
effective.
Anders Etzerodt, Associate Professor, PhD
Department of Biomedicine
Aarhus University, Denmark
| StreptaClick Precision | StreptaClick® Precision – 1 488 0.1 mg, StreptaClick® Precision – 1 647 0.1 mg, StreptaClick® Precision – 1 Azide 0.1 mg, StreptaClick® Precision – 1 Azide 0.5 mg, StreptaClick® Precision – 1 Azide 2 mg, StreptaClick® Precision – 1 DBCO 0.1 mg, StreptaClick® Precision – 1 DBCO 0.5 mg, StreptaClick® Precision – 1 DBCO 2 mg, StreptaClick® Precision – Oligo 0.1 mg, StreptaClick® Precision – Oligo 0.5 mg, StreptaClick® Precision – Oligo 2 mg |
|---|
Ordering information
| Cat no. | Product |
| 81Z0101-1 | StreptaClick® Precision - 1 Azide 0.1 mg |
| 81Z0501-1 | StreptaClick® Precision - 1 Azide 0.5 mg |
| 81Z2X01-1 | StreptaClick® Precision - 1 Azide 2 mg |
| 81D0101-1 | StreptaClick® Precision - 1 DBCO 0.1 mg |
| 81D0501-1 | StreptaClick® Precision - 1 DBCO 0.5 mg |
| 81D2X01-1 | StreptaClick® Precision - 1 DBCO 2 mg |
| 81N0101-1 | StreptaClick® Precision - 1 Oligo 0.1 mg |
| 81N0201-1 | StreptaClick® Precision - 1 Oligo 0.5 mg |
| 81N2X01-1 | StreptaClick® Precision - 1 Oligo 2 mg |
| 81F4880101-1 | StreptaClick® Precision - 1 488 0.1 mg |
| 81F6470101-1 | StreptaClick® Precision - 1 647 0.1 mg |
Please contact us to inquire about larger pack sizes and custom kit configurations.
RESOURCES
FLYER
StreptaClick® Precision flyer
USER MANUAL
User Manual StreptaClick® Precision - CuAAC
User Manual StreptaClick® Precision - SPAAC
PUBLICATIONS
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Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your BiotinPure™ Kit:
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Request a quote or order
Request a quote or order with payment by invoice by sending us an email via the button above. When ordering, please include information on the products you wish to order, organization, VAT number (only EU countries), delivery address, and invoice address. Please also state contact person and phone number for delivery by courier. We ship globally – a shipping cost of €50 is added to countries within the EU and €80 to all other countries. Upon request we can also charge in $USD for payments by invoice.Webshop
Orders can also be placed through our webshop via the form below. Once a product has been added a popup will appear with the choice of proceeding to checkout or adding additional products. The shopping cart icon at the top right corner also takes you to checkout. At checkout you will have the choice of secure payment by credit card via Stripe, Apple Pay, Google Pay or invoice. Shipping charges are added automatically. NB: VAT is not added to the final cost under Article 138 VAT directive (other EU countries) and 146 VAT directive (export to non-EU countries). Select your StreptaClick® Color Kit:
